Počet záznamů: 1
Helical coiling of metaphase chromatids
- 1.0572694 - ÚEB 2024 RIV US eng J - Článek v odborném periodiku
Kubalová, I. - Câmara, A. S. - Cápal, Petr - Beseda, Tomáš - Rouillard, J.-M. - Krause, G. M. - Holušová, Kateřina - Toegelová, Helena - Himmelbach, A. - Stein, N. - Houben, A. - Doležel, Jaroslav - Mascher, M. - Šimková, Hana - Schubert, V.
Helical coiling of metaphase chromatids.
Nucleic Acids Research. Roč. 51, č. 6 (2023), s. 2641-2654. ISSN 0305-1048. E-ISSN 1362-4962
Grant CEP: GA ČR(CZ) GC18-14450J; GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: MITOTIC CHROMOSOME STRUCTURE * SPIRAL STRUCTURE * TRADESCANTIA-REFLEXA
Obor OECD: Plant sciences, botany
Impakt faktor: 14.9, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1093/nar/gkad028
Chromatids of mitotic chromosomes were suggested to coil into a helix in early cytological studies and this assumption was recently supported by chromosome conformation capture (3C) sequencing. Still, direct differential visualization of a condensed chromatin fibre confirming the helical model was lacking. Here, we combined Hi-C analysis of purified metaphase chromosomes, biopolymer modelling and spatial structured illumination microscopy of large fluorescently labeled chromosome segments to reveal the chromonema-a helically-wound, 400 nm thick chromatin thread forming barley mitotic chromatids. Chromatin from adjacent turns of the helix intermingles due to the stochastic positioning of chromatin loops inside the chromonema. Helical turn size varies along chromosome length, correlating with chromatin density. Constraints on the observable dimensions of sister chromatid exchanges further supports the helical chromonema model.
Trvalý link: https://hdl.handle.net/11104/0343302
Název souboru Staženo Velikost Komentář Verze Přístup 2023_Kubalova_NUCLEIC ACIDS RESEARCH_2641_____@@@.pdf 2 12.3 MB Jiná povolen
Počet záznamů: 1