Study of rons formation in aquaeous solutions using dielectric barrier discharge and its bactericidal effects

0572504 - ÚVGZ 2024 CZ eng A - Abstrakt
Lišková, Kateřina - Čechová, L. - Krčma, F. - Kozáková, Z. - Červený, Jan
Study of rons formation in aquaeous solutions using dielectric barrier discharge and its bactericidal effects.
XXII. Workshop of Biophysical Chemists and Electrochemists Book of Abstracts. Brno: Masaryk University Press, 2022 - (Trnková, L.). s. 36-37
[XXII. Workshop of Biophysical Chemists and Electrochemists. 29.06.2022-29.06.2022, Brno]
Institucionální podpora: RVO:86652079
Klíčová slova: cold plasma * liquid activation * oxidative stress * bactericidal effect * bacteria
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

The recent trend of cold plasma liquid activation research comprises an increasing number of relevant publications dealing with a variety of devices and applications. Plasma activated liquids (PAL) contain species responsible for oxidative stress in bacterial cells. The most important in case of bactericidal effects is believed to be hydrogen peroxide. H2O2 itself isn’t able to cause much damage in the bacterial cell, however the secondary reaction between H2O2 and cellular Fe2+ ions create hydroxyl radicals, which are extremely reactive species that directly oxidize all cellular biomolecules. When H2O2 concentration is high enough, the oxidation will provide lethal effects. The bacterial cells can be also damaged by a nitrosative stress, which is in the case of PAL mainly caused by nitrites at low pH values. The acidification of NO2– produces a complex mixture of nitrogen oxides as well as nitrous acid, which is unstable and is spontaneously decomposed to produce NO and nitrogen dioxide. Chemical and bactericidal effects induced by plasma in aqueous solutions upon the dielectric barrier discharge (DBD) in air were investigated. For plasma treatment, distilled water and a fertilizer (Start-r, Mills, USA) were used. Inactivation of Escherichia Coli TOP10 strain was determined in dependence on pH and chemical changes induced in the studied solutions. Effect of PAL preparation time on the production of H2O2, NO2– and NO3– was studied, the concentrations of RONS were determined spectrophotometrically.
Trvalý link: https://hdl.handle.net/11104/0343164