Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein

0572379 - BC 2024 RIV US eng J - Článek v odborném periodiku
Bianchini, F. - Crivelli, V. - Abernathy, M. R. - Guerra, C. - Palus, Martin - Muri, J. - Marcotte, H. - Piralla, A. - Pedotti, M. - De Gasparo, R. - Simonelli, L. - Matkovic, M. - Toscano, C. - Biggiogero, M. - Calvaruso, V. - Svoboda, Pavel - Rincon, T. C. - Fava, T. - Podesvova, L. - Shanbhag, A. A. - Celoria, A. - Sgrignani, J. - Štefánik, M. - Hönig, Václav - Prančlová, Veronika - Michalčíková, Tereza - Procházka, Jan - Guerrini, G. - Mehn, D. - Clabbattini, A. - Sedláček, Radislav - Růžek, Daniel … celkem 46 autorů
Human neutralizing antibodies to cold linear epitopes and subdomain 1 of the SARS-CoV-2 spike glycoprotein.
SCI IMMUNOL. Roč. 8, č. 81 (2023), č. článku eade0958. ISSN 2470-9468. E-ISSN 2470-9468
Grant CEP: GA MŠMT(CZ) LX22NPO5103; GA ČR(CZ) GF21-05445L; GA ČR GF22-17139K; GA MŠMT(CZ) LM2018126
Výzkumná infrastruktura: Czech-BioImaging II - 90129
Institucionální podpora: RVO:60077344 ; RVO:68378050
Klíčová slova: SARS-CoV-2 * glycoprotein * epitopes
Obor OECD: Virology; Immunology (UMG-J)
Impakt faktor: 24.8, rok: 2022
Způsob publikování: Open access
https://www.science.org/doi/10.1126/sciimmunol.ade0958

Emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants diminishes the efficacy of vaccines and antiviral monoclonal antibodies. Continued development of immunotherapies and vaccine immunogens resilient to viral evolution is therefore necessary. Using coldspot-guided antibody discovery, a screening approach that focuses on portions of the virus spike glycoprotein that are both functionally relevant and averse to change, we identified human neutralizing antibodies to highly conserved viral epitopes. Antibody fp.006 binds the fusion peptide and cross-reacts against coronaviruses of the four genera, including the nine human coronaviruses, through recognition of a conserved motif that includes the S2 ' site of proteolytic cleavage. Antibody hr2.016 targets the stem helix and neutralizes SARS-CoV-2 variants. Antibody sd1.040 binds to subdomain 1, synergizes with antibody rbd.042 for neutralization, and, similar to fp.006 and hr2.016, protects mice expressing human angiotensin-converting enzyme 2 against infection when present as a bispecific antibody. Thus, coldspot-guided antibody discovery reveals donor-derived neutralizing antibodies that are crossreactive with Orthocoronavirinae, including SARS-CoV-2 variants.
Trvalý link: https://hdl.handle.net/11104/0343705