Diagnosis of Aspergillosis in Horses

0571794 - MBÚ 2024 RIV CH eng J - Článek v odborném periodiku
Dobiáš, R. - Jahn, P. - Tóthová, K. - Dobešová, O. - Višňovská, D. - Patil, Rutuja Hiraji - Škríba, Anton - Jaworská, P. - Škorič, M. - Podojil, L. - Kantorová, M. - Mrázek, J. - Krejčí, E. - Stevens, D. A. - Havlíček, Vladimír
Diagnosis of Aspergillosis in Horses.
Journal of Fungi. Roč. 9, č. 2 (2023), č. článku 161. E-ISSN 2309-608X
Grant CEP: GA ČR(CZ) GA21-17044S
Institucionální podpora: RVO:61388971
Klíčová slova: equine aspergillosis * invasive pulmonary aspergillosis * bronchoalveolar lavage fluid * tracheal wash * serum * galactomannan * (1,3)-beta-D-glucan * fungal secondary metabolites * equine herpes virus-5 * equine asthma * guttural pouch
Obor OECD: Microbiology
Impakt faktor: 4.7, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2309-608X/9/2/161

Invasive pulmonary aspergillosis (IPA) may be a rare cause of granulomatous pneumonia in horses. The mortality of IPA is almost 100%, direct diagnostic tools in horses are needed. Bronchoalveolar lavage fluid (BALF) and serum samples were collected from 18 horses, including individuals suffering from IPA (n = 1), equine asthma (EA, n = 12), and 5 healthy controls. Serum samples were collected from another 6 healthy controls. Samples of BALF (n = 18) were analyzed for Aspergillus spp. DNA, fungal galactomannan (GM), ferricrocin (Fc), triacetylfusarinin C (TafC), and gliotoxin (Gtx). Analysis of 24 serum samples for (1,3)-beta-D-glucan (BDG) and GM was performed. Median serum BDG levels were 131 pg/mL in controls and 1142 pg/mL in IPA. Similar trends were observed in BALF samples for GM (Area under the Curve (AUC) = 0.941) and DNA (AUC = 0.941). The fungal secondary metabolite Gtx was detected in IPA BALF and lung tissue samples (86 ng/mL and 2.17 ng/mg, AUC = 1).
Trvalý link: https://hdl.handle.net/11104/0342693