Porcine oocytes matured in a chemically defined medium are transcriptionally active

0571786 - ÚŽFG 2024 RIV US eng J - Článek v odborném periodiku
Murín, Matěj - Němcová, Lucie - Bártková, Alexandra - Gad, Ahmed - Lucas-Hahn, A. - Strejček, F. - Procházka, Radek - Laurinčík, Jozef
Porcine oocytes matured in a chemically defined medium are transcriptionally active.
Theriogenelogy. Roč. 203, JUN 23 (2023), s. 89-98. ISSN 0093-691X. E-ISSN 1879-3231
Grant CEP: GA MŠMT EF15_003/0000460
Institucionální podpora: RVO:67985904
Klíčová slova: Porcine oocyte * Maturation in vitro * Transcriptome * Chemically defined medium
Obor OECD: Developmental biology
Impakt faktor: 2.8, rok: 2022
Způsob publikování: Omezený přístup
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=6037039682

The statement that fully-grown porcine oocytes (oocytes from follicles with diameter from 3 to 6 mm) are transcriptionally quiescent is not as strongly supported as it was before. Currently, we know that there is a difference between the transcription profile of germinal vesicle (GV) and metaphase II (MII) oocytes. The goal of our study was to compare the transcription profile of GV, germinal vesicle break-down (GVBD), metaphase I (MI), and MII oocytes matured in the chemically defined medium FLI. Oocytes were sequenced, and the results were subsequently validated using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We detected multiple differentially transcribed mRNAs, of which many were upregulated. Among them we found mRNAs necessary for protein production, mitochondrial functions and cytoplasmic maturation. Collectively, these data support the hypothesis that transcription activity in fully-grown porcine oocytes is necessary for key processes during their successful maturation in vitro in a chemically defined maturation medium.(c) 2023 Published by Elsevier Inc.
Trvalý link: https://hdl.handle.net/11104/0343122