Bacterial, but not fungal, communities show spatial heterogeneity in European beech (Fagus sylvatica L.) deadwood

0571650 - MBÚ 2024 RIV US eng J - Článek v odborném periodiku
Bosch, Jason - Nemethová, Ema - Tláskal, Vojtěch - Brabcová, Vendula - Baldrian, Petr
Bacterial, but not fungal, communities show spatial heterogeneity in European beech (Fagus sylvatica L.) deadwood.
FEMS Microbiology Ecology. Roč. 99, č. 4 (2023), č. článku fiad023. ISSN 0168-6496. E-ISSN 1574-6941
Grant CEP: GA ČR(CZ) GC21-09334J
Institucionální podpora: RVO:61388971
Klíčová slova: bacteria * deadwood * ecology * fungi * microbial communities * temperate forest * wood decay
Obor OECD: Microbiology
Impakt faktor: 4.2, rok: 2022
Způsob publikování: Open access
https://academic.oup.com/femsec/article/99/4/fiad023/7076322?login=true

Deadwood decomposition and other environmental processes mediated by microbial communities are generally studied with composite sampling strategies, where deadwood is collected from multiple locations in a large volume, that produce an average microbial community. In this study, we used amplicon sequencing to compare fungal and bacterial communities sampled with either traditional, composite samples, or small, 1 cm(3) cylinders from a discrete location within decomposing European beech (Fagus sylvatica L.) tree trunks. We found that bacterial richness and evenness is lower in small samples when compared to composite samples. There was no significant difference in fungal alpha diversity between different sampling scales, suggesting that visually defined fungal domains are not restricted to a single species. Additionally, we found that composite sampling may obscure variation in community composition and this affects the understanding of microbial associations that are detected. For future experiments in environmental microbiology, we recommend that scale is explicitly considered as a factor and properly selected to correspond with the questions asked. Studies of microbial functions or associations may require samples to be collected at a finer scale than is currently practised.
Trvalý link: https://hdl.handle.net/11104/0342811