Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds

0571563 - BÚ 2024 RIV TW eng J - Článek v odborném periodiku
Šoch, J. - Šonka, J. - Ponert, Jan
Acid scarification as a potent treatment for an in vitro germination of mature endozoochorous Vanilla planifolia seeds.
Botanical Studies. Roč. 64, č. 1 (2023), č. článku 9. E-ISSN 1999-3110
Institucionální podpora: RVO:67985939
Klíčová slova: seed germination * Vanilla planifolia * acid scarification * orchid * in vitro cultivation * hydrochloric acid * HCl * calcium hypochlorite * sulfuric acid
Obor OECD: Plant sciences, botany
Impakt faktor: 3.4, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1186/s40529-023-00374-z

Vanilla planifolia is the most widely cultivated species of vanilla with high economic importance. However, seed germination under artificial conditions is difficult and yields low germination percentages. The seeds are adapted to endozoochorous dispersal, and we therefore tried to simulate the conditions in the digestive tract by acid scarification of seeds.Immature seeds lacking dormancy, used as a control, showed the highest germination percentage. Among the treatments tested for mature seeds, the hydrochloric acid treatments were significantly the best in breaking dormancy and inducing germination, irrespective of the acid concentration and the presence of pepsin. Conventional treatment with a hypochlorite solution induced much lower germination percentage. Sulphuric acid at concentration 50% was too strong and caused damage to the seeds. Important factor is also high cultivation temperature 30 degrees C as there was nearly no germination at 25 degrees C.Our protocol significantly improves the efficiency of generative propagation of vanilla and allows for significantly higher germination percentages than previously described. The strongly positive effect of hydrochloric acid may be related to the adaptation of seeds to endozoochorous dispersal.
Trvalý link: https://hdl.handle.net/11104/0352052