The Ixodes ricinus salivary gland proteome during feeding and B. Afzelii infection: New avenues for an anti-tick vaccine

0571515 - BC 2024 RIV GB eng J - Článek v odborném periodiku
Klouwens, M.J. - Trentelman, J.J.A. - Barriales, D. - Ersoz, J. - Azkargorta, M. - Elortza, F. - Šíma, Radek - Hajdušek, Ondřej - Cortazar, J.T. - Corcuera, I. E. - Colstrup, E. - Nayak, A. - Ruiz, I.M. - Rodriguez, H. - Nijhof, A.M. - Anguita, J. - Hovius, J.W.R.
The Ixodes ricinus salivary gland proteome during feeding and B. Afzelii infection: New avenues for an anti-tick vaccine.
Vaccine. Roč. 41, č. 12 (2023), s. 1951-1960. ISSN 0264-410X. E-ISSN 1873-2518
Grant CEP: GA MŠMT(CZ) EF16_019/0000759; GA MZd(CZ) NU20-05-00396; GA ČR(CZ) GA22-30920S
Institucionální podpora: RVO:60077344
Klíčová slova: Borrelia afzelii * Proteomics * Ixodes ricinus * Salivary glands * Feeding * Antitick vaccines
Obor OECD: Microbiology
Impakt faktor: 5.5, rok: 2022
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S0264410X23001299?via%3Dihub

Introduction: Borrelia burgdorferi sensu lato, the causative agents of Lyme borreliosis, are transmitted by Ixodes ticks. Tick saliva proteins are instrumental for survival of both the vector and spirochete and have been investigated as targets for vaccine targeting the vector. In Europe, the main vector for Lyme borre-liosis is Ixodes ricinus, which predominantly transmits Borrelia afzelii. We here investigated the differen-tial production of I. ricinus tick saliva proteins in response to feeding and B. afzelii infection.Method: Label-free Quantitative Proteomics and Progenesis QI software was used to identify, compare, and select tick salivary gland proteins differentially produced during tick feeding and in response to B. afzelii infection. Tick saliva proteins were selected for validation, recombinantly expressed and used in both mouse and guinea pig vaccination and tick-challenge studies.Results: We identified 870 I. ricinus proteins from which 68 were overrepresented upon 24-hours of feed-ing and B. afzelii infection. Selected tick proteins were successfully validated by confirming their expres-sion at the RNA and native protein level in independent tick pools. When used in a recombinant vaccine formulation, these tick proteins significantly reduced the post-engorgement weights of I. ricinus nymphs in two experimental animal models. Despite the reduced ability of ticks to feed on vaccinated animals, we observed efficient transmission of B. afzelii to the murine host.Conclusion: Using quantitative proteomics, we identified differential protein production in I. ricinus sali-vary glands in response to B. afzelii infection and different feeding conditions. These results provide novel insights into the process of I. ricinus feeding and B. afzelii transmission and revealed novel candidates for an anti-tick vaccine.(c) 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Trvalý link: https://hdl.handle.net/11104/0345557