Capillary isoelectric focusing-The role of markers of isoelectric point and recent applications in the field

0571276 - ÚIACH 2024 RIV NL eng J - Článek v odborném periodiku
Duša, Filip - Kubesová, Anna - Šalplachta, Jiří - Moravcová, Dana
Capillary isoelectric focusing-The role of markers of isoelectric point and recent applications in the field.
TrAC-Trends in Analytical Chemistry. Roč. 162, MAY (2023), č. článku 117018. ISSN 0165-9936. E-ISSN 1879-3142
Grant CEP: GA MZd(CZ) NU22-05-00110; GA MV VI20172020069; GA ČR(CZ) GA22-00236S
Institucionální podpora: RVO:68081715
Klíčová slova: capillary * isoelectric focusing * isoelectric point
Obor OECD: Analytical chemistry
Impakt faktor: 13.1, rok: 2022
Způsob publikování: Omezený přístup

Capillary isoelectric focusing (cIEF) is a very powerful separation technique. However, successful implementation of this technique in various fields of science requires a few prerequisites including a repeatable formation of pH gradient and a set of internal standards (i.e., markers of isoelectric point) to track the actual profile of the pH gradient. Overview of the available isoelectric point markers and their syntheses is presented in this review. Moreover, recent applications of cIEF for analysis of complex larger structures such as viruses, vesicles, and bacterial cells are also reviewed.(c) 2023 Elsevier B.V. All rights reserved.
Trvalý link: https://hdl.handle.net/11104/0342538