OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision

0570770 - ÚMG 2024 RIV CH eng J - Článek v odborném periodiku
Lindovský, Jiří - Pálková, Marcela - Symkina, Viktoriia - Raishbrook, Miles Joseph - Procházka, Jan - Sedláček, Radislav
OCT and ERG Techniques in High-Throughput Phenotyping of Mouse Vision.
Genes. Roč. 14, č. 2 (2023), č. článku 294. E-ISSN 2073-4425
Grant CEP: GA MŠMT(CZ) LM2018126; GA MŠMT EF18_046/0015861
Institucionální podpora: RVO:68378050
Klíčová slova: phenotyping * vision * oct * erg * impc
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 3.5, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2073-4425/14/2/294

The purpose of the study is to demonstrate coherent optical tomography and electroretinography techniques adopted from the human clinical practice to assess the morphology and function of the mouse retina in a high-throughput phenotyping environment. We present the normal range of wild-type C57Bl/6NCrl retinal parameters in six age groups between 10 and 100 weeks as well as examples of mild and severe pathologies resulting from knocking out a single protein-coding gene. We also show example data obtained by more detailed analysis or additional methods useful in eye research, for example, the angiography of a superficial and deep vascular complex. We discuss the feasibility of these techniques in conditions demanding a high-throughput approach such as the systemic phenotyping carried out by the International Mouse Phenotyping Consortium.
Trvalý link: https://hdl.handle.net/11104/0342109