Axonal Transport

0569530 - BTÚ 2024 RIV US eng M - Část monografie knihy
Puttrich, Verena - Rohlena, Jakub - Braun, Marcus - Lánský, Zdeněk
In Vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport.
Axonal Transport. New York: Springer Protocols, 2022 - (Vagnoni, A.), s. 533-546. ISBN 978-1-0716-1992-6
Institucionální podpora: RVO:86652036
Klíčová slova: Adaptor proteins * Interference reflection microscopy * Kinesin-1 * Mitochondria * Molecular motors * Motility assay * TIRF microscopy * trak
Obor OECD: Biochemistry and molecular biology
https://link.springer.com/protocol/10.1007/978-1-0716-1990-2_28

Intracellular trafficking of organelles driven by molecular motors underlies essential cellular processes. Mitochondria, the powerhouses of the cell, are one of the major cargoes of molecular motors. Efficient distribution of mitochondria ensures cellular fitness while defects in this process contribute to severe pathologies, such as neurodegenerative diseases. Reconstitution of the mitochondrial microtubule-based transport in vitro in a bottom-up approach provides a powerful tool to investigate the mitochondrial trafficking machinery in a controlled environment in the absence of complex intracellular interactions. In this chapter, we describe the procedures for achieving such reconstitution of mitochondrial transport.
Trvalý link: https://hdl.handle.net/11104/0342658