Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants

0569333 - MBÚ 2024 RIV CH eng J - Článek v odborném periodiku
Šplíchal, Igor - Donovan, S. M. - Kindlová, Zdislava - Straňák, Z. - Neužil Bunešová, V. - Šinkora, Marek - Poláková, Kateřina - Valášková, Barbora - Šplíchalová, Alla
Release of HMGB1 and Toll-like Receptors 2, 4, and 9 Signaling Are Modulated by Bifidobacterium animalis subsp. lactis BB-12 and Salmonella Typhimurium in a Gnotobiotic Piglet Model of Preterm Infants.
International Journal of Molecular Sciences. Roč. 24, č. 3 (2023), č. článku 2329. E-ISSN 1422-0067
Grant CEP: GA ČR GA13-14736S; GA ČR(CZ) GA20-03282S
Institucionální podpora: RVO:61388971
Klíčová slova: Bifidobacterium animalis subsp * lactis BB-12 * Salmonella Typhimurium * high mobility group box 1 * Toll-like receptors * tight junction proteins * mucin * intestinal barrier * inflammatory cytokines * immunodeficient host
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 5.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1422-0067/24/3/2329

Gnotobiotic (GN) animals with defined microbiota allow us to study host-microbiota and microbiota-microbiota interferences. Preterm germ-free (GF) piglets were mono-associated with probiotic Bifidobacterium animalis subsp. lactis BB-12 (BB12) to ameliorate/prevent the consequences of infection with the Salmonella Typhimurium strain LT2 (LT2). Goblet cell density, expression of Toll-like receptors (TLRs) 2, 4, and 9, high mobility group box 1 (HMGB1), interleukin (IL)-6, and IL-12/23p40 were analyzed to evaluate the possible modulatory effect of BB12. BB12 prevented an LT2-induced decrease of goblet cell density in the colon. TLRs signaling modified by LT2 was not influenced by the previous association with BB12. The expression of HMGB1, IL-6, and IL12/23p40 in the jejunum, ileum, and colon and their levels in plasma were all decreased by BB12, but these changes were not statistically significant. In the colon, differences in HMGB1 distribution between the GF and LT2 piglet groups were observed. In conclusion, the mono-association of GF piglets with BB12 prior to LT2 infection partially ameliorated the inflammatory response to LT2 infection.
Trvalý link: https://hdl.handle.net/11104/0342819