Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing

0569324 - FZÚ 2024 RIV US eng J - Článek v odborném periodiku
Savvulidi, F.G. - Ptáček, M. - Málková, A. - Kratochvílová, Irena - Šimek, Daniel - Martinez-Pastor, F. - Stadnik, L.
Inhibition of extracellular ice crystals growth for testing the cryodamaging effect of intracellular ice in a model of ram sperm ultra-rapid freezing.
Journal of Applied Animal Research. Roč. 51, č. 1 (2023), s. 182-192. ISSN 0971-2119. E-ISSN 0974-1844
Grant CEP: GA MŠMT(CZ) EF16_019/0000760
Grant ostatní: OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institucionální podpora: RVO:68378271
Klíčová slova: cryodamage * extracellular ice * synthetic ice blocker * X-ray diffraction
Obor OECD: Biophysics
Impakt faktor: 1.4, rok: 2022
Způsob publikování: Open access

We aimed to test the common belief, that as the sperm cell is the smallest cell in an organism and, therefore, it contains a small amount of intracellular water, the intracellular ice should not strongly affect the sperm cell during the cryopreservation. To investigate the effect of intracellular ice, we developed a novel approach of ram spermatozoa ultra-rapid freezing with the inhibition of the growth of extracellular ice crystals during freezing. In our approach, ram sperm was supplemented with cell-impermeable synthetic ice blocker 1,4-Cyclohexanediol, leaving a sample unsupplemented as a control.
Trvalý link: https://hdl.handle.net/11104/0342071