Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes

0568949 - BFÚ 2024 RIV GB eng J - Článek v odborném periodiku
Dobrovodský, Daniel - Daňhel, Aleš - Mothes-Martin, E. - Pratviel, G. - Renčiuk, Daniel - Fojta, Miroslav
Interfacial behaviour of oligodeoxynucleotides prone to G-quadruplex formation on negatively charged electrode surface monitored by electrochemical probes.
Electrochimica acta. Roč. 442, FEB 20 2023 (2023), č. článku 141878. ISSN 0013-4686. E-ISSN 1873-3859
Grant CEP: GA MŠMT EF15_003/0000477; GA ČR(CZ) GA22-11252S
Institucionální podpora: RVO:68081707
Klíčová slova: dna * Adsorption * G-quadruplex * Porphyrin * Voltammetry
Obor OECD: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Impakt faktor: 6.6, rok: 2022
Způsob publikování: Omezený přístup
https://www.sciencedirect.com/science/article/abs/pii/S0013468623000658?via%3Dihub

Development of analytical tools enabling discrimination amongst various structures of nucleic acids attracts interest due to diverse biological functions and prospective biotechnological applications of the latter. In this work, two electrochemically active compounds, copper porphyrin complex Cu-TMPy2PP and base-modified deoxyguanosine triphosphate conjugate bearing 3-nitrophenyl moiety (dGNPTP), were used as probes to study electrode potential effects on oligodeoxynucleotides (ODNs) of various sequences and abilities to form secondary structures (including unstructured single strands, duplexes, guanine quadruplexes, i-motifs) at the surface of hanging mercury drop electrode (HMDE). Our experiments revealed formation of less dense adsorbed layers by G-rich ODNs with the propensity to fold into G-quadruplexes (G4), compared to layers formed by other studied sequences. Strikingly, the G4-forming ODNs were significantly more susceptible to desorption from the HMDE surface during its polarization to highly negative potentials compared to other ODNs. Negative potential-induced desorption of the ODNs from HMDE was further confirmed by gel electrophoresis. Application of the electro-chemical probes offers a useful method for further research of DNA conformations and transitions taking place in solution and at electrically charged surfaces.
Trvalý link: https://hdl.handle.net/11104/0344339