Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation

0568847 - ÚIACH 2024 RIV CH eng J - Článek v odborném periodiku
Lehotská Mikušová, M. - Bušová, M. - Tulinská, J. - Mašánová, V. - Lisková, A. - Uhnáková, I. - Dusinská, M. - Krivošíková, Z. - Rollerova, E. - Aláčová, R. - Wsólová, L. - Horváthová, M. - Szabová, M. - Lukan, N. - Večeřa, Zbyněk - Coufalík, Pavel - Křůmal, Kamil - Alexa, Lukáš - Thon, V. - Piler, P. - Buchtová, Marcela - Vrlíková, Lucie - Moravec, Pavel - Galanda, D. - Mikuška, Pavel
Titanium Dioxide Nanoparticles Modulate Systemic Immune Response and Increase Levels of Reduced Glutathione in Mice after Seven-Week Inhalation.
Nanomaterials. Roč. 13, č. 4 (2023), č. článku 767. E-ISSN 2079-4991
Grant CEP: GA ČR(CZ) GA20-02203S; GA ČR(CZ) GBP503/12/G147
Institucionální podpora: RVO:68081715 ; RVO:67985904 ; RVO:67985858
Klíčová slova: titanium dioxide nanoparticles * nanoparticle inhalation * immunotoxicity
Obor OECD: Analytical chemistry; Physical chemistry (UCHP-M)
Impakt faktor: 5.3, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2079-4991/13/4/767

Titanium dioxide nanoparticles (TiO2 NPs) are used in a wide range of applications. Although inhalation of NPs is one of the most important toxicologically relevant routes, experimental studies on potential harmful effects of TiO2 NPs using a whole-body inhalation chamber model are rare. In this study, the profile of lymphocyte markers, functional immunoassays, and antioxidant defense markers were analyzed to evaluate the potential adverse effects of seven-week inhalation exposure to two different concentrations of TiO2 NPs (0.00167 and 0.1308 mg TiO2/m3) in mice. A dose-dependent effect of TiO2 NPs on innate immunity was evident in the form of stimulated phagocytic activity of monocytes in low-dose mice and suppressed secretory function of monocytes (IL-18) in high-dose animals. The effect of TiO2 NPs on adaptive immunity, manifested in the spleen by a decrease in the percentage of T-cells, a reduction in T-helper cells, and a dose-dependent decrease in lymphocyte cytokine production, may indicate immunosuppression in exposed mice. The dose-dependent increase in GSH concentration and GSH/GSSG ratio in whole blood demonstrated stimulated antioxidant defense against oxidative stress induced by TiO2 NP exposure.
Trvalý link: https://hdl.handle.net/11104/0340115