Preparative 3D Printed Device for the Short DNA Fragment Separation

0568258 - ÚIACH 2023 CH eng A - Abstrakt
Hrušková, Helena - Řemínek, Roman - Foret, František
Preparative 3D Printed Device for the Short DNA Fragment Separation.
Separations. MDPI. Roč. 10, č. 109 (2023), s. 86-87. E-ISSN 2297-8739.
[APCE-CECE-ITP-IUPAC 2022. 06.11.2022-10.11.2022, Angkor Wat]
Grant CEP: GA TA ČR(CZ) FW02020209
Institucionální podpora: RVO:68081715
Klíčová slova: 3D-printed device * blood plasma samples * DNA fragments
Obor OECD: Analytical chemistry
https://www.mdpi.com/2297-8739/10/2/109

We developed a new 3D-printed device for preparative precleaning blood plasma samples from larger DNA fragments. Such a sample adjustment should increase the quality of data obtained from subsequent PCR, where only particular short DNA fragments are considered to be biomarkers of the cancer disease. In our study, the device was designed, fabricated by 3D-printing from the polymeric material HD PLA, and tested. A method based on electromigration separation techniques for the preparative separation of short DNA fragments from larger fragments has been developed and partially validated.

Trvalý link: https://hdl.handle.net/11104/0339588