Selective and reversible disruption of mitochondrial inner membrane protein complexes by lipophilic cations

0567732 - BC 2024 RIV NL eng J - Článek v odborném periodiku
Kafková, A. - Tilokani, L. - Trčka, F. - Šrámková, V. - Vancová, Marie - Bílý, Tomáš - Nebesářová, Jana - Prudent, J. - Trnka, J.
Selective and reversible disruption of mitochondrial inner membrane protein complexes by lipophilic cations.
Mitochondrion. Roč. 68, JAN (2023), s. 60-71. ISSN 1567-7249. E-ISSN 1872-8278
Grant CEP: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF16_013/0001775
Institucionální podpora: RVO:60077344
Klíčová slova: Mitochondria * Inner mitochondrial membrane * Lipophilic cations * MitoQ * Mitochondrial dynamics * Respiratory chain
Obor OECD: Cell biology
Impakt faktor: 4.4, rok: 2022
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/pii/S1567724922001039?via%3Dihub

Triphenylphosphonium (TPP) derivatives are commonly used to target chemical into mitochondria. We show that alkyl-TPP cause reversible, dose-and hydrophobicity-dependent alterations of mitochondrial morphology and function and a selective decrease of mitochondrial inner membrane proteins including subunits of the res-piratory chain complexes, as well as components of the mitochondrial calcium uniporter complex. The treatment with alkyl-TPP resulted in the cleavage of the pro-fusion and cristae organisation regulator Optic atrophy-1. The structural and functional effects of alkyl-TPP were found to be reversible and not merely due to loss of membrane potential. A similar effect was observed with the mitochondria-targeted antioxidant MitoQ.
Trvalý link: https://hdl.handle.net/11104/0344203