Reducing the number of accepted species in Aspergillus series Nigri

0567685 - MBÚ 2023 RIV NL eng J - Článek v odborném periodiku
Bian, C. - Kusuya, Y. - Sklenář, František - D’hooge, E. - Yaguchi, T. - Ban, S. - Visagie, C.M. - Houbraken, J. - Takahashi, H. - Hubka, Vít
Reducing the number of accepted species in Aspergillus series Nigri.
Studies in Mycology. Roč. 102, č. 1 (2022), s. 95-132. ISSN 0166-0616. E-ISSN 1872-9797
Grant CEP: GA MZd(CZ) NU21-05-00681
Institucionální podpora: RVO:61388971
Klíčová slova: Aspergillus luchuensis * Aspergillus niger * Aspergillus tubingensis * clinical fungi * indoor fungi * infraspecific variability * multigene phylogeny, * multispecies coalescence model * ochratoxin A * species delimitation
Obor OECD: Microbiology
Impakt faktor: 16.5, rok: 2022
Způsob publikování: Open access
https://www.ingentaconnect.com/content/wfbi/sim/2022/00000102/00000001/art00004#

The Aspergillus series Nigri contains biotechnologically and medically important species. They can produce hazardous mycotoxins, which is relevant due to the frequent occurrence of these species on foodstuffs and in the indoor environment. The taxonomy of the series has undergone numerous rearrangements, and currently, there are 14 species accepted in the series, most of which are considered cryptic. Species-level identifications are, however, problematic or impossible for many isolates even when using DNA sequencing or MALDI-TOF mass spectrometry, indicating a possible problem in the definition of species limits or the presence of undescribed species diversity. To re-examine the species boundaries, we collected DNA sequences from three phylogenetic markers (benA, CaM and RPB2) for 276 strains from series Nigri and generated 18 new whole-genome sequences. With the threegene dataset, we employed phylogenetic methods based on the multispecies coalescence model, including four single-locus methods (GMYC, bGMYC, PTP and bPTP) and one multilocus method (STACEY). From a total of 15 methods and their various settings, 11 supported the recognition of only three species corresponding to the three main phylogenetic lineages: A. niger, A. tubingensis and A. brasiliensis. Similarly, recognition of these three species was supported by the GCPSR approach (Genealogical Concordance Phylogenetic Species Recognition) and analysis in DELINEATE software. We also showed that the phylogeny based on benA, CaM and RPB2 is suboptimal and displays significant differences from a phylogeny constructed using 5 752 single-copy orthologous proteins, therefore, the results of the delimitation methods may be subject to a higher than usual level of uncertainty. To overcome this, we randomly selected 200 genes from these genomes and performed ten independent STACEY analyses, each with 20 genes.
Trvalý link: https://hdl.handle.net/11104/0338910