The Inability of Ex Vivo Expanded Mesenchymal Stem/Stromal Cells to Survive in Newborn Mice and to Induce Transplantation Tolerance

0567576 - ÚEM 2023 RIV US eng J - Článek v odborném periodiku
Holáň, Vladimír - Echalar, Barbora - Palacká, Kateřina - Kössl, Jan - Boháčová, Pavla - Porubská, Bianka - Krulová, Magdaléna - Javorková, Eliška - Zajícová, Alena
The Inability of Ex Vivo Expanded Mesenchymal Stem/Stromal Cells to Survive in Newborn Mice and to Induce Transplantation Tolerance.
Stem Cell Reviews and Reports. Roč. 18, č. 7 (2022), s. 2365-2375. ISSN 2629-3269. E-ISSN 2629-3277
Grant CEP: GA ČR(CZ) GA19-02290S
Institucionální podpora: RVO:68378041
Klíčová slova: mesenchymal stem cells * newborn mice * immunological reactivity * cytokines * skin grafts * transplantation tolerance
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 4.8, rok: 2022
Způsob publikování: Open access
https://link.springer.com/article/10.1007/s12015-022-10363-7

An encounter of the developing immune system with an antigen results in the induction of immunological areactivity to this antigen. In the case of transplantation antigens, the application of allogeneic hematopoietic cells induces a state of neonatal transplantation tolerance. This tolerance depends on the establishment of cellular chimerism, when allogeneic cells survive in the neonatally treated recipient. Since mesenchymal stem/stromal cells (MSCs) have been shown to have low immunogenicity and often survive in allogeneic recipients, we attempted to use these cells for induction of transplantation tolerance. Newborn (less than 24 h old) C57BL/6 mice were injected intraperitoneally with 5 x 10(6) adipose tissue-derived MSCs isolated from allogeneic donors and the fate and survival of these cells were monitored. The impact of MSC application on the proportion of cell populations of the immune system and immunological reactivity was assessed. In addition, the survival of skin allografts in neonatally treated recipients was tested. We found that in vitro expanded MSCs did not survive in neonatal recipients, and the living MSCs were not detected few days after their application. Furthermore, there were no significant changes in the proportion of individual immune cell populations including CD4(+) cell lineages, but we detected an apparent shift to the production of Th1 cytokines IL-2 and IFN-gamma in neonatally treated mice. However, skin allografts in the MSC-treated recipients were promptly rejected. These results therefore show that in vitro expanded MSCs do not survive in neonatal recipients, but induce a cytokine imbalance without induction of transplantation tolerance.
Trvalý link: https://hdl.handle.net/11104/0338822