pH-Dependent disruption of giant polymer vesicles: a step towards biomimetic membranes

0567447 - ÚMCH 2024 RIV GB eng J - Článek v odborném periodiku
Sincari, Vladimir - Jäger, Eliezer - Loureiro, Kahynna Cavalcante - Vragović, Martina - Hofmann, E. - Schlenk, M. - Filipová, Marcela - Rydvalová, Eliška - Štěpánek, Petr - Hrubý, Martin - Förster, S. - Jäger, Alessandro
pH-Dependent disruption of giant polymer vesicles: a step towards biomimetic membranes.
Polymer Chemistry. Roč. 14, č. 4 (2023), s. 443-451. ISSN 1759-9954. E-ISSN 1759-9962
Grant CEP: GA ČR(CZ) GJ20-13946Y; GA ČR(CZ) GJ20-15077Y; GA ČR(CZ) GA21-04166S
Institucionální podpora: RVO:61389013
Klíčová slova: polymer * vesicles * giant
Obor OECD: Polymer science
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Omezený přístup
https://pubs.rsc.org/en/content/articlelanding/2023/PY/D2PY01229D

Giant unilamellar polymer vesicles (GUPVs) capable of selectively delivering protected payloads into intracellular environments and releasing them in stimuli-triggered, precise spatially and temporally controlled manners are attractive bioactive cargo delivery tools. Herein, we present highly size-defined and monodisperse (42.1 ± 1.2, 60.5 ± 1.0, 80.4 ± 1.4 and 97.9 ± 1.2 μm in diameter, respectively) pH-responsive giant GUPVs prepared via microfluidic droplet generation using a flow-focusing poly(dimethylsiloxane) (PDMS)-based microfluidic device. Poly(ethylene oxide)-block-poly[2-(diisopropylamino)ethyl methacrylate] (PEO-b-PDPA) is a pH-responsive polymer that was synthesized via reversible addition–fragmentation chain-transfer (RAFT) polymerization and used in combination with poly(ethylene oxide)-block-poly(1,2-butadiene) (PEO-b-PBD) to produce homogeneous pH-responsive giant GUPVs. To demonstrate the spatiotemporal control provided by this approach, we studied in detail the pH-responsiveness of GUPVs according to the disruption and release of dye cargo under distinct acidic conditions using fluorescence confocal microscopy. This approach can be utilized to fabricate pH-responsive delivery systems for various active compounds, microreactors, and artificial organelles, thereby paving the way towards cell mimicry.

Trvalý link: https://hdl.handle.net/11104/0342244