0567217 - ÚEM 2023 RIV CH eng J - Článek v odborném periodiku
Ambrož, Antonín - Rössner ml., Pavel - Rössnerová, Andrea - Hoňková, Kateřina - Milcová, Alena - Pastorková, Anna - Kléma, J. - Pulkrabová, J. - Pařízek, O. - Vondrašková, V. - Zelenka, J. - Vrzáčková, N. - Schmuczerová, J. - Topinka, Jan - Šrám, Radim
Oxidative Stress and Antioxidant Response in Populations of the Czech Republic Exposed to Various Levels of Environmental Pollutants.
International Journal of Environmental Research and Public Health. Roč. 19, č. 6 (2022), č. článku 3609. ISSN 1661-7827. E-ISSN 1660-4601
Grant CEP: GA MŠMT(CZ) LM2018124; GA MŠMT(CZ) LM2018133; GA MŠMT(CZ) EF16_013/0001821; GA MŠMT(CZ) EF16_019/0000798
Institucionální podpora: RVO:68378041
Klíčová slova: oxidative damage * DNA * lipids * antioxidant response * environmental factors * POPs
Obor OECD: Public and environmental health
Impakt faktor: 4.614, rok: 2021 ; AIS: 0.866, rok: 2021
Způsob publikování: Open access
Web výsledku:
https://www.mdpi.com/1660-4601/19/6/3609DOI: https://doi.org/10.3390/ijerph19063609

We aimed to identify the variables that modify levels of oxidatively damaged DNA and lipid peroxidation in subjects living in diverse localities of the Czech Republic (a rural area, a metropolitan locality, and an industrial region). The sampling of a total of 126 policemen was conducted twice in two sampling seasons. Personal characteristics, concentrations of particulate matter of aerodynamic diameter <2.5 mu m and benzo[a]pyrene in the ambient air, activities of antioxidant mechanisms (superoxide dismutase, catalase, glutathione peroxidase, and antioxidant capacity), levels of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, and IL-6), concentrations of persistent organic pollutants in blood plasma, and urinary levels of polycyclic aromatic hydrocarbon metabolites were investigated as parameters potentially affecting the markers of DNA oxidation (8-oxo-7,8-dihydro-2 -deoxyguanosine) and lipid peroxidation (15-F2t-isoprostane). The levels of oxidative stress markers mostly differed between the localities in the individual sampling seasons. Multivariate linear regression analysis revealed IL-6, a pro-inflammatory cytokine, as a factor with the most pronounced effects on oxidative stress parameters. The role of other variables, including environmental pollutants, was minor. In conclusion, our study showed that oxidative damage to macromolecules was affected by processes related to inflammation, however, we did not identify a specific environmental factor responsible for the pro-inflammatory response in the organism.
Trvalý link: https://hdl.handle.net/11104/0338504