Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae)

0566784 - BC 2024 RIV DE eng J - Článek v odborném periodiku
Hejníčková, Martina - Dalíková, M. - Zrzavá, Magda - Marec, František - Lorite, P. - Montiel, E. E.
Accumulation of retrotransposons contributes to W chromosome differentiation in the willow beauty Peribatodes rhomboidaria (Lepidoptera: Geometridae).
Scientific Reports. Roč. 13, č. 1 (2023), č. článku 534. ISSN 2045-2322. E-ISSN 2045-2322
Grant CEP: GA ČR(CZ) GA20-13784S; GA MŠMT(CZ) LM2018131
Grant ostatní: GA ČR(CZ) GJ20-20650Y; Ministerstvo školství, mládeže a tělovýchovy - GA MŠk(CZ) LM2018140; GA JU(CZ) 013/20220/P
Výzkumná infrastruktura: e-INFRA CZ - 90140
Institucionální podpora: RVO:60077344
Klíčová slova: sex chromosome evolution * retrotrasposons * FISH mapping
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.nature.com/articles/s41598-023-27757-3.pdf

The W chromosome of Lepidoptera is typically gene-poor, repeat-rich and composed of heterochromatin. Pioneering studies investigating this chromosome reported an abundance of mobile elements. However, the actual composition of the W chromosome varies greatly between species, as repeatedly demonstrated by comparative genomic hybridization (CGH) or fluorescence in situ hybridization (FISH). Here we present an analysis of repeats on the W chromosome in the willow beauty, Peribatodes rhomboidaria (Geometridae), a species in which CGH predicted an abundance of W-enriched or W-specific sequences. Indeed, comparative analysis of male and female genomes using RepeatExplorer identified ten putative W chromosome-enriched repeats, most of which are LTR or LINE mobile elements. We analysed the two most abundant: PRW LINE-like and PRW Bel-Pao. The results of FISH mapping and bioinformatic analysis confirmed their enrichment on the W chromosome, supporting the hypothesis that mobile elements are the driving force of W chromosome differentiation in Lepidoptera. As the W chromosome is highly underrepresented in chromosome-level genome assemblies of Lepidoptera, this recently introduced approach, combining bioinformatic comparative genome analysis with molecular cytogenetics, provides an elegant tool for studying this elusive and rapidly evolving part of the genome.
Trvalý link: https://hdl.handle.net/11104/0349825