Hard tissues on a chip – development of a platform for generating 3D bone and cartilage cultures

0566670 - ÚIACH 2023 CZ eng A - Abstrakt
Killinger, Michael - Szotkovská, Tereza - Václavek, Tomáš - Buchtová, Marcela - Klepárník, Karel
Hard tissues on a chip – development of a platform for generating 3D bone and cartilage cultures.
Czech Chemical Society Symposium Series. "Amerika" 21st, č. 20 (2022), s. 21-22. ISSN 2336-7202.
[Mezioborové setkání mladých biologů, biochemiků a chemiků /21./. 16.05.2022-19.05.2022, Milovy]
Grant CEP: GA ČR(CZ) GA20-00726S
Institucionální podpora: RVO:68081715 ; RVO:67985904
Klíčová slova: 3D Bone In Vitro Models * microfluidics
Obor OECD: Analytical chemistry

In this study, we developed a polydimethylsiloxane (PDMS) microfluidic platform suitable for 3D bone and cartilaginous cells cultivation. The proper fabrication and preparation led to the development of homogenous 3D cell cultures of MC3T3-E1 osteoblast cells. Moreover, the system was used to study primary chondrocyte differentiation while using WNT inhibitors. These protocols were compared to standard cultivation methods such as hanging drops to reflect the advantages of microfluidic platforms.
Trvalý link: https://hdl.handle.net/11104/0337982