Arthrobacter polaris sp. nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil

0566474 - MBÚ 2023 RIV GB eng J - Článek v odborném periodiku
Vodickova, P. - Šuman, J. - Benešová, E. - Strejček, M. - Neumann-Schaal, M. - Cajthaml, Tomáš - Rídl, Jakub - Pajer, P. - Ulbrich, P. - Uhlík, O. - Lipovová, P.
Arthrobacter polaris sp. nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
International Journal of Systematic and Evolutionary Microbiology. Roč. 72, č. 10 (2022), č. článku 005541. ISSN 1466-5026. E-ISSN 1466-5034
Grant CEP: GA MŠMT(CZ) LM2018131
Institucionální podpora: RVO:61388971 ; RVO:68378050
Klíčová slova: Antarctica * Arthrobacter * cold-adapted microorganisms * Micrococcaceae * whole-genome sequencing
Obor OECD: Microbiology; Microbiology (UMG-J)
Impakt faktor: 2.8, rok: 2022
Způsob publikování: Omezený přístup
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005541

An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic. Cells with a size of 0.65–0.9×1.2–1.7 µm have a flagellar motile apparatus and exhibit a rod–coccus growth cycle. Optimal growth conditions were observed at 15–20 °C, pH 7.0 and NaCl concentration up to 0.5% (w/v) in the medium. The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77% to Arthrobacter glacialis NBRC 113092T. Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the ‘Arthrobacter psychrolactophilus group’. The G+C content of genomic DNA was 58.95mol%. The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15% and 77.38%, respectively. The digital DNA–DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6%. The polar lipid profile was composed mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The predominant cellular fatty acids were anteiso-C15:0 (75%) and anteiso-C17:0 (15.2%). Menaquinone MK-9(H2) (86.4%) was the major respiratory quinone in strain C1-1T. The peptidoglycan type was determined as A3α (l-Lys–l-Ala3, A11.6). Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp. nov.
Trvalý link: https://hdl.handle.net/11104/0337797