Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study

0566276 - MBÚ 2023 RIV GB eng J - Článek v odborném periodiku
Deissová, T. - Cvanová, M. - Kala, Z. - Jirásková Zákostelská, Zuzana - Dolina, J. - Kunovský, L. - Kroupa, R. - Pavlovský, Z. - Lipový, B. - Daněk, Z. - Izakovičová Hollá, L. - Urban, O. - Navrátil, V. - Lischke, R. - Harustiak, T. - Grolich, T. - Procházka, V. - Slabý, O. - Borilova Linhartova, P.
Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study.
Disease Markers. Roč. 2022, Aug 31 (2022), č. článku 8790748. ISSN 0278-0240. E-ISSN 1875-8630
GRANT EU: European Commission(CZ) 857560
Výzkumná infrastruktura: RECETOX RI - 90121
Institucionální podpora: RVO:61388971
Klíčová slova: epidermal growth factor * polymorphisms * gene expression * reflux esophagitis * barrett's esophagus * esophageal adenocarcinoma
Obor OECD: Medical biotechnology related ethics
Impakt faktor: 3.464, rok: 2021
Způsob publikování: Open access
https://www.hindawi.com/journals/dm/2022/8790748/

The epidermal growth factor (EGF) and its receptor (EGFR) gene-gene interactions were shown to increase the susceptibility to esophageal cancer. However, the role of the EGF/EGFR pathway in the development of gastroesophageal reflux disease (GERD) and its complications (reflux esophagitis (RE), Barrett's esophagus (BE), and esophageal adenocarcinoma (EAC)) remains unclear. This association study is aimed at investigating functional EGF and EGFR gene polymorphisms, their mRNA expression in esophageal tissues, and EGF plasma levels in relation to RE, BE, and EAC development in the Central European population. 301 patients with RE/BE/EAC (cases) as well as 98 patients with nonerosive reflux disease (NERD) and 8 healthy individuals (controls) were genotyped for +61 A>G EGF (rs4444903) and +142285 G>A EGFR (rs2227983) polymorphisms using the TaqMan quantitative polymerase chain reaction (qPCR). In random subgroups, the EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in esophageal tissue with and without endoscopically visible pathological changes, and the EGF plasma levels were determined by enzyme-linked immunosorbent assay. None of the genotyped SNPs nor EGF-EGFR genotype interactions were associated with RE, BE, or EAC development (p>0.05). Moreover, mRNA expression of neither EGF nor EGFR differed between samples of the esophageal tissue with and without endoscopically visible pathology (p>0.05) nor between samples from patients with different diagnoses, i.e., RE, BE, or EAC (p>0.05). Nevertheless, the lower EGF mRNA expression in carriers of combined genotypes AA +61 EGF (rs4444903) and GG +142285 EGFR (rs2227983, p<0.05) suggests a possible direct/indirect effect of EGF-EGFR gene interactions on EGF gene expression. In conclusion, EGF and EGFR gene variants and their mRNA/protein expression were not associated with RE, BE or EAC development in the Central European population.
Trvalý link: https://hdl.handle.net/11104/0337664