Plectin-mediated cytoskeletal crosstalk controls cell tension and cohesion in epithelial sheets

0566275 - ÚMG 2023 RIV US eng J - Článek v odborném periodiku
Přechová, Magdalena - Adamová Outlá, Zuzana - Schweizer, A. - Maninová, Miloslava - Bauer, A. - Kah, D. - Meier-Menches, S.M. - Wiche, G. - Fabry, B. - Gregor, Martin
Plectin-mediated cytoskeletal crosstalk controls cell tension and cohesion in epithelial sheets.
Journal of Cell Biology. Roč. 221, č. 3 (2022), č. článku e202105146. ISSN 0021-9525. E-ISSN 1540-8140
Grant CEP: GA MZd NV17-31538A; GA ČR GA21-21736S; GA MŠMT(CZ) LQ1604; GA MŠMT(CZ) LM2018129; GA MŠMT LTC17063; GA MŠMT(CZ) LM2015062; GA MŠMT LO1419; GA MŠMT(CZ) LM2015040; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) EF18_046/0016045
GRANT EU: European Commission(XE) CA15214 - EuroCellNet
Institucionální podpora: RVO:68378050
Klíčová slova: Mechanical properties * Vimentin Network * Actin * Architecture * Desmoplakin * Stability * Filaments * Dynamics * Linkage * Key
Obor OECD: Cell biology
Impakt faktor: 7.8, rok: 2022
Způsob publikování: Open access
https://rupress.org/jcb/article/221/3/e202105146/212995/Plectin-mediated-cytoskeletal-crosstalk-controls

The coordinated interplay of cytoskeletal networks critically determines tissue biomechanics and structural integrity. Here, we show that plectin, a major intermediate filament-based cytolinker protein, orchestrates cortical cytoskeletal networks in epithelial sheets to support intercellular junctions. By combining CRISPR/Cas9-based gene editing and pharmacological inhibition, we demonstrate that in an F-actin-dependent context, plectin is essential for the formation of the circumferential keratin rim, organization of radial keratin spokes, and desmosomal patterning. In the absence of plectin-mediated cytoskeletal cross-linking, the aberrant keratin-desmosome (DSM)-network feeds back to the actin cytoskeleton, which results in elevated actomyosin contractility. Also, by complementing a predictive mechanical model with Forster resonance energy transfer-based tension sensors, we provide evidence that in the absence of cytoskeletal cross-linking, major intercellular junctions (adherens junctions and DSMs) are under intrinsically generated tensile stress. Defective cytoarchitecture and tensional disequilibrium result in reduced intercellular cohesion, associated with general destabilization of plectin-deficient sheets upon mechanical stress.
Trvalý link: https://hdl.handle.net/11104/0337746