Is Renal beta-Adrenergic-WNK4-NCC Pathway Important in Salt Hypertension of Dahl Rats?

0565778 - FGÚ 2023 RIV CZ eng J - Článek v odborném periodiku
Zicha, Josef - Hojná, Silvie - Vaňourková, Z. - Kopkan, L. - Vaněčková, Ivana
Is Renal beta-Adrenergic-WNK4-NCC Pathway Important in Salt Hypertension of Dahl Rats?
Physiological Research. Roč. 68, č. 6 (2019), s. 873-882. ISSN 0862-8408. E-ISSN 1802-9973
Grant CEP: GA MZd(CZ) NV15-25396A
Institucionální podpora: RVO:67985823
Klíčová slova: sympathetic nervous system * beta-adrenergic blockade * renal sodium excretion * NCC cotransporter * hydrochlorothiazide * serine/threonine-protein kinase WNK4
Obor OECD: Physiology (including cytology)
Impakt faktor: 1.655, rok: 2019
Způsob publikování: Open access
http://www.biomed.cas.cz/physiolres/pdf/2019/68_873.pdf

In 2011 Fujita and coworkers proposed that beta-adrenergic stimulation causes decreased serine/threonine-protein kinase WNK4 transcription leading to the activation of Na-CI cotransporter (NCC) which participates in salt sensitivity and salt hypertension development in rodents. The aim of our study was to investigate whether the above hypothesis is also valid for salt hypertension of Dahl rats, which are characterized by high sympathetic tone and abnormal renal sodium handling. Male 8-week-old salt-sensitive (SS/Jr) and salt-resistant (SR/Jr) Dahl rats were fed either low-salt diet (LS, 0.4 % NaCI) or high-salt diet (HS, 4 % NaCI) for 6 weeks. Half of the animals on either diet were chronically treated with non-selective 8-blocker propranolol (100 mg/kg/day). At the end of the experiment diuresis and sodium excretion were measured prior and after hydrochlorothiazide injection (HCTZ, 10 mg/kg i.p.). Furthermore, blood pressure (BP), heart rate (HR), sympathetic (pentolinium 5 mg/kg i.v.) and NO-dependent (L-NAME 30 mg/kg i.v.) BP components were determined. Chronic HS diet feeding increased BP through sympathoexcitation in SS/Jr but not in SR/Jr rats. Concomitant propranolol treatment did not lower BP in either experimental group. Under the conditions of low salt intake HCTZ increased diuresis, natriuresis and fractional sodium excretion in SR/Jr but not in SS/Jr rats. HS diet feeding attenuated renal response to HCT in SR/Jr rats, whereas no HCTZ effect was observed in SS/Jr rats fed HS diet. Propranolol treatment did not modify diuresis or natriuresis in any experimental group. In conclusions, our present data do not support the idea on the essential importance of renal beta-adrenergic-WNK4-NCC pathway in pathogenesis and/or maintenance of salt hypertension in Dahl rats.
Trvalý link: https://hdl.handle.net/11104/0337271