Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor

0565771 - MBÚ 2023 RIV GB eng J - Článek v odborném periodiku
Lopez Marin, M.A. - Šuman, J. - Jani, K. - Ulbrich, P. - Cajthaml, Tomáš - Pajer, P. - Wolf, J. - Neumann-Schaal, M. - Strejček, M. - Uhlík, O.
Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor.
International Journal of Systematic and Evolutionary Microbiology. Roč. 72, č. 8 (2022), č. článku 005467. ISSN 1466-5026. E-ISSN 1466-5034
Grant CEP: GA MŠMT(CZ) LM2018131
Institucionální podpora: RVO:61388971
Klíčová slova: compost soil * extracellular organic matter * resuscitation-promoting factor * soil bacteria * Sphingosinicellaceae * whole genome average nucleotide identity
Obor OECD: Microbiology
Impakt faktor: 2.8, rok: 2022
Způsob publikování: Omezený přístup
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005467

An orange-golden iridescent culture, designated A1X5R2(T), was isolated from a compost soil suspension which was amended with Micrococcus luteus NCTC 2665(T) culture supernatant. The cells were non-motile, Gram-stain-negative, 0.4-0.5 mu m wide and 0.7-1.4 mu m long. The 16S rRNA-based phylogenetic and whole-genome analyses revealed that strain A1X5R2(T) forms a distinct lineage within the family Sphingosinicellaceae and is closely related to members of the genus Sphingoaurantiacus ( S. capsulatus , 93.04 % similarity, and S. polygranulatus , 92.77 %). The organism grew at 22-47 degrees C (optimal at 37 degrees C), salinity <3 % (optimal at 1.5 %) and at pH 7. The major respiratory quinone was ubiquinone-10, but a small quantity of ubiquinone-9 was also detected The major polyamine was homospermidine, but a small quantity of putrescine was also detected. The strain contained C-18:1 omega 7c, C-16:0, C-16:1 omega 7c and C-18:0 as the major fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, sphingoglycolipid, diphosphatidylglycerol, two unidentified phospholipids and three unidentified amino lipids. The DNA G+C content was 64.9 mol%. According to the results of phylogenetic and phylogenomic analyses, as well as its physiological characteristics, strain A2X5R2(T) represents the type species of a novel genus within the family Sphingosinicellaceae . The name Pedomonas mirosovicensis gen. nov., sp. nov. is proposed, with the type strain being A1X5R2(T) (=NCCB 100839(T)=DSM 112829(T)).
Trvalý link: https://hdl.handle.net/11104/0337707