Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells

0565699 - FGÚ 2023 RIV DE eng J - Článek v odborném periodiku
Zárubová, Jana - Hasani-Sadrabadi, M. M. - Norris, S. C. P. - Majedi, F. S. - Xiao, C. - Kasko, A. M. - Li, S.
Cell-Taxi: Mesenchymal Cells Carry and Transport Clusters of Cancer Cells.
Small. Roč. 18, č. 50 (2022), č. článku 2203515. ISSN 1613-6810. E-ISSN 1613-6829
Institucionální podpora: RVO:67985823
Klíčová slova: circulating tumor cell clusters * collective cell migration * extracellular vesicles * micropatterned hydrogels * spheroid migration assay
Obor OECD: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)
Impakt faktor: 13.3, rok: 2022
Způsob publikování: Omezený přístup
https://doi.org/10.1002/smll.202203515

Cell clusters that collectively migrate from primary tumors appear to be far more potent in forming distant metastases than single cancer cells. A better understanding of the collective cell migration phenomenon and the involvement of various cell types during this process is needed. Here, an in vitro platform based on inverted-pyramidal microwells to follow and quantify the collective migration of hundreds of tumor cell clusters at once is developed. These results indicate that mesenchymal stromal cells (MSCs) or cancer-associated fibroblasts (CAFs) in the heterotypic tumor cell clusters may facilitate metastatic dissemination by transporting low-motile cancer cells in a Rac-dependent manner and that extracellular vesicles secreted by mesenchymal cells only play a minor role in this process. Furthermore, in vivo studies show that cancer cell spheroids containing MSCs or CAFs have faster spreading rates. These findings highlight the active role of co-traveling stromal cells in the collective migration of tumor cell clusters and may help in developing better-targeted therapies.
Trvalý link: https://hdl.handle.net/11104/0337218