The relation between protein adsorption and hemocompatibility of antifouling polymer brushes

0564084 - ÚMCH 2023 RIV DE eng J - Článek v odborném periodiku
Riedelová, Zuzana - de los Santos Pereira, Andres - Svoboda, Jan - Pop-Georgievski, Ognen - Májek, P. - Pečánková, K. - Dyčka, F. - Rodriguez-Emmenegger, C. - Riedel, Tomáš
The relation between protein adsorption and hemocompatibility of antifouling polymer brushes.
Macromolecular Bioscience. Roč. 22, č. 11 (2022), č. článku 2200247. ISSN 1616-5187. E-ISSN 1616-5195
Grant CEP: GA ČR(CZ) GA20-10845S; GA ČR(CZ) GF21-16729K
Grant ostatní: AV ČR(CZ) MSM200502001
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institucionální podpora: RVO:61389013
Klíčová slova: antifouling surfaces * hemocompatibility * MS identification
Obor OECD: Biophysics
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Omezený přístup
https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200247

Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.

Trvalý link: https://hdl.handle.net/11104/0336175