Počet záznamů: 1
Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward
- 1.0564055 - MBÚ 2023 RIV NL eng J - Článek v odborném periodiku
Lekberg, Y. - Baath, E. - Frostegård, Å. - Hammer, E. - Hedlund, K. - Jansa, Jan - Kaiser, C. - Ramsey, P. W. - Řezanka, Tomáš - Rousk, J. - Wallander, H. - Welc, M. - Olsson, P. A.
Fatty acid 16:1 omega 5 as a proxy for arbuscular mycorrhizal fungal biomass: current challenges and ways forward.
Biology and Fertility of Soils. Roč. 58, č. 8 (2022), s. 835-842. ISSN 0178-2762. E-ISSN 1432-0789
Institucionální podpora: RVO:61388971
Klíčová slova: PLFA 16:1 omega 5 * NLFA 16:1 omega 5 * Arbuscular mycorrhizal fungi * Biomass * Gram-negative bacteria
Obor OECD: Microbiology
Impakt faktor: 6.5, rok: 2022
Způsob publikování: Open access
https://link.springer.com/article/10.1007/s00374-022-01670-9
Fatty acid biomarkers have emerged as a useful tool to quantify biomass of various microbial groups. Here we focus on the frequent use of the fatty acid 16:1 omega 5 as a biomarker for arbuscular mycorrhizal (AM) fungi in soils. We highlight some issues with current applications of this method and use several examples from the literature to show that the phospholipid fatty acid (PLFA) 16:1 omega 5 can occur in high concentrations in soils where actively growing AM fungi are absent. Unless the study includes a control where the contribution of other microbes can be estimated, we advocate for the use of the neutral lipid fatty acid (NLFA) 16:1 omega 5. This biomarker has higher specificity, is more responsive to shifts in AM fungal biomass, and quantification can be conducted along with PLFA analysis without doubling analytical efforts. We conclude by contrasting various methods used to measure AM fungal biomass in soil and highlight future research needs to optimize fatty acid analyses.
Trvalý link: https://hdl.handle.net/11104/0335803
Počet záznamů: 1