Pertussis toxin suppresses dendritic cell-mediated delivery of B. pertussis into lung-draining lymph nodes

0562934 - ÚMG 2023 RIV US eng J - Článek v odborném periodiku
Klímová, M. - Holubová, J. - Streparola, Gaia - Tomala, J. - Brazdilova, L. - Staněk, O. - Bumba, L. - Šebo, P.
Pertussis toxin suppresses dendritic cell-mediated delivery of B. pertussis into lung-draining lymph nodes.
PLoS Pathogens. Roč. 18, č. 6 (2022), č. článku e1010577. ISSN 1553-7366. E-ISSN 1553-7374
Grant CEP: GA MŠMT(CZ) LM2018129; GA MŠMT(CZ) EF18_046/0016045; GA MŠMT(CZ) LM2018126; GA MŠMT EF18_046/0015861; GA MŠMT ED2.1.00/19.0395; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:68378050
Klíčová slova: Adenylate-cyclase toxin * respiratory-tract colonization * Bordetella-pertissis * neutrophil recruitment * antigen presentation * ADP-ribosylation * immune-response * RTX proteins * in vitro * infection
Obor OECD: Virology
Impakt faktor: 6.7, rok: 2022
Způsob publikování: Open access
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1010577

Author summaryOf the three classical Bordetella species causing respiratory infections in mammals, only the human-specialized whooping cough agent B. pertussis produces the pertussis toxin (PT) as its major virulence factor. Human pertussis is an acute respiratory illness and the pleiotropic activities of pertussis toxin account for the characteristic systemic manifestations of the disease, such as hyperleukocytosis, histamine sensitization, hyperinsulinemia, or inflammatory lung pathology. We found that PT activity inhibits the migration of infected dendritic cells from the lungs into the draining mediastinal lymph nodes (mLNs). This prevents mLN infection by bacteria evading from migratory cells and delivery of bacterial antigens into mLNs. As a result, the induction of adaptive serum antibody responses to infection is delayed. We thus propose that PT action serves to create a time window for proliferation of B. pertussis on airway mucosa to facilitate transmission of the pathogen among humans.
Trvalý link: https://hdl.handle.net/11104/0336324