Migration of repetitive DNAs during evolution of the permanent translocation heterozygosity in the oyster plant (Tradescantia section Rhoeo)

0562816 - ÚEB 2023 RIV DE eng J - Článek v odborném periodiku
Golczyk, H. - Hřibová, Eva - Doležel, Jaroslav - Cuadrado, A. - Garbsch, F. - Greiner, S. - Janeczko, M. - Szklarczyk, M. - Masłyk, M. - Kubiński, K.
Migration of repetitive DNAs during evolution of the permanent translocation heterozygosity in the oyster plant (Tradescantia section Rhoeo).
Chromosoma. Roč. 131, č. 3 (2022), s. 163-173. ISSN 0009-5915. E-ISSN 1432-0886
Grant CEP: GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: Inversions * Permanent translocation heterozygosity * Rabl configuration * Repetitive DNA * Tradescantia spathacea * Translocations
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 1.6, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1007/s00412-022-00776-1

Due to translocation heterozygosity for all chromosomes in the cell complement, the oyster plant (Tradescantia spathacea) forms a complete meiotic ring. It also shows Rabl-arrangement at interphase, featured by polar centromere clustering. We demonstrate that the pericentromeric regions of the oyster plant are homogenized in concert by three subtelomeric sequences: 45S rDNA, (TTTAGGG)n motif, and TSrepI repeat. The Rabl-based clustering of pericentromeric regions may have been an excellent device to combine the subtelomere-pericentromere sequence migration (via inversions) with the pericentromere-pericentromere DNA movement (via whole arm translocations) that altogether led to the concerted homogenization of all the pericentromeric domains by the subtelomeric sequences. We also show that the repetitive sequence landscape of interstitial chromosome regions contains many loci consisting of Arabidopsis-type telomeric sequence or of TSrepI repeat, and it is extensively heterozygous. However, the sequence arrangement on some chromosomal arms suggest segmental inversions that are fully or partially homozygous, a fact that could be explained if the inversions started to create linkages already in a bivalent-forming ancestor. Remarkably, the subterminal TSrepI loci reside exclusively on the longer arms that could be due to sharing sequences between similarly-sized chromosomal arms in the interphase nucleus. Altogether, our study spotlights the supergene system of the oyster plant as an excellent model to link complex chromosome rearrangements, evolution of repetitive sequences, and nuclear architecture.
Trvalý link: https://hdl.handle.net/11104/0335010