Hyaluronan/zinc oxide nanocomposite-based membrane: preparation, characterization, and in vitro and in vivo evaluation

0562639 - ÚMCH 2023 RIV US eng J - Článek v odborném periodiku
Abdel-Rahman, Rasha M. - Franková, J. - Sklenarova, R. - Kaprálková, Ludmila - Kelnar, Ivan - Abdel-Mohsen, Abdellatif M.
Hyaluronan/zinc oxide nanocomposite-based membrane: preparation, characterization, and in vitro and in vivo evaluation.
ACS Applied Polymer Materials. Roč. 4, č. 10 (2022), s. 7723-7738. ISSN 2637-6105. E-ISSN 2637-6105
Grant CEP: GA ČR(CZ) GA19-06065S
Institucionální podpora: RVO:61389013
Klíčová slova: hyaluronan membrane * zinc oxide nanoparticles * mechanical properties
Obor OECD: Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics
Impakt faktor: 5, rok: 2022
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/acsapm.2c01296

Hyaluronic acid (HA) has attracted the attention of scientists for its application in many medical fields owing to its intrinsic properties such as biodegradability, biocompatibility, and nontoxicity. Poor stability and antibacterial activity are the most significant drawbacks of using HA in preparing a wound dressing. The wound dressing should be able to swell and absorb exudates from the wound, as well as possess antibacterial activity. Herein, we successfully prepared an HA-based antibacterial wound dressing that can swell more than 500 times and with good mechanical properties. HA was used both as reducing and stabilizing agents, and zinc oxide nanoparticles (ZnO-NPs) were incorporated via in situ synthesis. The uniform spherical shape of ZnO-NPs (50 ± 10 nm) was synthesized and assembled on a hyaluronan/polyvinyl alcohol matrix (HA/PVA), forming stable and evenly distributed ZnO-NPs, which interacted with the HA/PVA. Such a hybrid nanostructure prevented ZnO-NPs from dropping off the PVA/HA network and thus minimized the toxicity of NPs. Concerning the slow release of NPs, the nanocomposite membrane still exhibited significant antibacterial activity against different types of bacteria (G+/−). The ZnO-NPs/HA/PVA composite membrane allows the attachment and growth of normal human dermal fibroblasts and human primary osteogenic sarcoma (Saos-2) without exhibiting toxicity. In vivo measurements showed that the nanocomposite PVA/HA/ZnO-NP membrane promoted infected wound healing compared with the control sample. The results obtained suggest that the investigated nanocomposite has reasonable potential as an antibacterial wound dressing material.
Trvalý link: https://hdl.handle.net/11104/0335276