Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants

0562283 - ÚEB 2023 RIV IE eng J - Článek v odborném periodiku
Abdouli, D. - Plačková, Lenka - Doležal, Karel - Bettaieb, T. - Werbrouck, S.
Topolin cytokinins enhanced shoot proliferation, reduced hyperhydricity and altered cytokinin metabolism in Pistacia vera L. seedling explants.
Plant Science. Roč. 322, SEP (2022), č. článku 111360. ISSN 0168-9452. E-ISSN 1873-2259
Grant CEP: GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Endogenous cytokinin * meta-topolin riboside * Pistachio * Shoot tip necrosis
Obor OECD: Biochemical research methods
Impakt faktor: 5.2, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1016/j.plantsci.2022.111360

The effect of 10 µM meta-topolin (mT) and meta-topolin riboside (mTR) on in vitro proliferation and anomalies of Pistacia vera L. were evaluated and compared to that of 6-benzylaminopurine (BA). The highest proliferation rate (15.6) was recorded in the mT-medium, with a value 6 times higher than in BA-medium. Moreover, the lowest percentage of hyperhydric usable shoots (58,9%) and callus weight (46,9%) were found in mTR-treated shoots. Shoot tip as well as leaf necrosis were not influenced by cytokinin (CK) type. Image analysis was used to evaluate photosynthetic efficiency as well as anthocyanin index. Photosynthesis was more efficient with BA and mTR but the higher anthocyanin accumulation in BA-treated shoots suggests more stress. Endogenous CKs and their metabolites were determined in seedlings and, for the first time, the metabolism of exogenous BA, mT and mTR was studied in pistachio. The stimulating effect on cis-zeatin and its riboside and the appearance of BA and traces of ortho-topolin and para-topolin as natural CKs are discussed. The quantitative and qualitative CK metabolite analyses provides some initial clues as to why topolin would be superior to BA in terms of proliferation rate and avoiding hyperhydricity and allowed a better understanding of the effect of exogenous administration of CK.
Trvalý link: https://hdl.handle.net/11104/0334633