Cadmium in the hyperaccumulating mushroom Thelephora penicillata: Intracellular speciation and isotopic composition

0561743 - ÚJF 2024 RIV NL eng J - Článek v odborném periodiku
Borovička, Jan - Sácký, J. - Kaňa, A. - Walenta, M. - Ackerman, L. - Braeuer, S. - Leonhardt, T. - Hršelová, H. - Goessler, W. - Kotrba, P.
Cadmium in the hyperaccumulating mushroom Thelephora penicillata: Intracellular speciation and isotopic composition.
Science of the Total Environment. Roč. 855, JAN (2023), č. článku 159002. ISSN 0048-9697. E-ISSN 1879-1026
Institucionální podpora: RVO:61389005
Klíčová slova: cadmium isotopes * double spike * fungi * hyperaccumulation * metal sequestration * Thermal Ionization Mass Spectrometry (TIMS)
Obor OECD: Environmental sciences (social aspects to be 5.7)
Impakt faktor: 9.8, rok: 2022
Způsob publikování: Omezený přístup
https://doi.org/10.1016/j.scitotenv.2022.159002

Thelephora penicillata is an ectomycorrhizal mushroom that can accumulate extraordinarily high concentrations of Cd, As, Cu, and Zn in its fruit-bodies. To better understand its element accumulation ability, we compared the element concentrations in T. penicillata with 10 distinct ectomycorrhizal mushroom species growing at the same site (Karlina Pila, Czech Republic). On average, T. penicillata accumulated 330, 2130, 26, and 4 times more Cd, As, Cu, and Zn, respectively, than other mushrooms. Size-exclusion chromatography and an electrophoretic analysis of T. penicillata cell extracts indicate that intracellular Cd may be present mainly in >1 kDa, presumably compartmentalized, Cd species, and partially binding with 6-kDa cysteinyl-containing peptide(s) resembling metallothioneins. The cadmium isotopic composition of mushroom fruit-bodies, soil digests, and soil extracts was investigated by thermal ionization mass spectrometry (TIMS) with double spike correction. The isotopic composition (δ114/110Cd) of ectomycorrhizal mushrooms from Karlina Pila varied in a wide range of −0.37 to +0.14 ‰. However, remarkably low δ114/110Cd values were observed in the majority of the investigated mushrooms when compared to the relatively homogeneous Cd isotopic composition of bulk soil (δ114/110Cd = +0.09 ‰) and the comparatively heavy isotopic composition of soil extracts (mean δ114/110Cd values of +0.11 ± 0.01 ‰ and +0.22 ± 0.01 ‰, depending on the extraction method). The isotopic composition of Cd hyperaccumulated in T. penicillata essentially matched the mycoavailable soil Cd fraction. However, most isotopic data indicates isotopic fractionation at the soil/fruit-body interface, which could be of environmental significance.
Trvalý link: https://hdl.handle.net/11104/0334266