Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions

0561123 - ÚŽFG 2023 RIV GB eng J - Článek v odborném periodiku
Bendová, B. - Mikula, Ondřej - Vošlajerová Bímová, Barbora - Čížková, D. - Daniszová, Kristina - Ďureje, L. - Hiadlovská, Zuzana - Macholán, Miloš - Martin, J.-F. - Piálek, J. - Schmiedová, L. - Kreisinger, J.
Divergent gut microbiota in two closely related house mouse subspecies under common garden conditions.
FEMS Microbiology Ecology. Roč. 98, č. 8 (2022), č. článku fiac078. ISSN 0168-6496. E-ISSN 1574-6941
Grant CEP: GA ČR GAP506/11/1792
Institucionální podpora: RVO:67985904
Klíčová slova: evolution * gut microbiome * Helicobacter
Obor OECD: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impakt faktor: 4.2, rok: 2022
Způsob publikování: Omezený přístup
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=90812963689

The gastrointestinal microbiota (GM) is considered an important component of the vertebrate holobiont. GM-host interactions influence the fitness of holobionts and are, therefore, an integral part of evolution. The house mouse is a prominent model for GM-host interactions, and evidence suggests a role for GM in mouse speciation. However, previous studies based on short 16S rRNA GM profiles of wild house mouse subspecies failed to detect GM divergence, which is a prerequisite for the inclusion of GM in Dobzhansky-Muller incompatibilities. Here, we used standard 16S rRNA GM profiling in two mouse subspecies, Mus musculus musculus and M. m. domesticus, including the intestinal mucosa and content of three gut sections (ileum, caecum, and colon). We reduced environmental variability by sampling GM in the offspring of wild mice bred under seminatural conditions. Although the breeding conditions allowed a contact between the subspecies, we found a clear differentiation of GM between them, in all three gut sections. Differentiation was mainly driven by several Helicobacters and two H. ganmani variants showed a signal of codivergence with their hosts. Helicobacters represent promising candidates for studying GM-host coadaptations and the fitness effects of their interactions.

Sequencing of bacterial 16S rRNA gene reveales differences in gut microbiota between two house mouse subspecies (mainly formed by genus Helicobacter), their gut segments, and between gut mucosa and content.
Trvalý link: https://hdl.handle.net/11104/0333814