Tomato growth promotion by the fungal endophytes Serendipita indica and Serendipita herbamans is associated with sucrose de-novo synthesis in roots and differential local and systemic effects on carbohydrate metabolisms and gene expression

0561107 - ÚVGZ 2023 RIV PT eng J - Článek v odborném periodiku
De Rocchis, V. - Jammer, A. - Camehl, I. - Franken, P. - Roitsch, Thomas
Tomato growth promotion by the fungal endophytes Serendipita indica and Serendipita herbamans is associated with sucrose de-novo synthesis in roots and differential local and systemic effects on carbohydrate metabolisms and gene expression.
Journal of Plant Physiology. Roč. 276, SEP (2022), č. článku 153755. ISSN 0176-1617. E-ISSN 1618-1328
Grant CEP: GA MŠMT(CZ) LO1415
Výzkumná infrastruktura: CzeCOS III - 90123
Institucionální podpora: RVO:86652079
Klíčová slova: Sebacinales * Plantfungus interaction * Carbohydrate metabolism * Tomato * Growth promotion * Sucrose
Obor OECD: Plant sciences, botany
Impakt faktor: 4.3, rok: 2022
Způsob publikování: Open access
https://www.sciencedirect.com/science/article/abs/pii/S0176161722001419

Plant growth-promoting and stress resilience-inducing root endophytic fungi represent an additional carbohy-drate sink. This study aims to test if such root endophytes affect the sugar metabolism of the host plant to divert the flow of resources for their purposes. Fresh and dry weights of roots and shoots of tomato (Solanum lyco-persicum) colonised by the closely related Serendipita indica and Serendipita herbamans were recorded. Plant carbohydrate metabolism was analysed by measuring sugar levels, by determining activity signatures of key enzymes of carbohydrate metabolism, and by quantifying mRNA levels of genes involved in sugar transport and turnover. During the interaction with the tomato plants, both fungi promoted root growth and shifted shoot biomass from stem to leaf tissues, resulting in increased leaf size. A common effect induced by both fungi was the inhibition of phosphofructokinase (PFK) in roots and leaves. This glycolytic-pacing enzyme shows how the glycolysis rate is reduced in plants and, eventually, how sugars are allocated to different tissues. Sucrose phosphate synthase (SPS) activity was strongly induced in colonised roots. This was accompanied by increased SPS-A1 gene expression in S. herbamans-colonised roots and by increased sucrose amounts in roots colonised by S. indica. Other enzyme activities were barely affected by S. indica, but mainly induced in leaves of S. herbamans- colonised plants and decreased in roots. This study suggests that two closely related root endophytic fungi differentially influence plant carbohydrate metabolism locally and systemically, but both induce a similar in-crease in plant biomass. Notably, both fungal endophytes induce an increase in SPS activity and, in the case of S. indica, sucrose resynthesis in roots. In leaves of S. indica-colonised plants, SWEET11b expression was enhanced, thus we assume that excess sucrose was exported by this transporter to the roots.
Trvalý link: https://hdl.handle.net/11104/0334032