Počet záznamů: 1
Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells
- 1.0560854 - ÚOCHB 2023 RIV DE eng J - Článek v odborném periodiku
Sigaeva, A. - Hochstetter, A. - Bouyim, S. - Chipaux, M. - Štejfová, Miroslava - Cígler, Petr - Schirhagl, R.
Single-Particle Tracking and Trajectory Analysis of Fluorescent Nanodiamonds in Cell-Free Environment and Live Cells.
Small. Roč. 18, č. 39 (2022), č. článku 2201395. ISSN 1613-6810. E-ISSN 1613-6829
Grant CEP: GA MŠMT EF16_026/0008382
Výzkumná infrastruktura: CANAM II - 90056
Institucionální podpora: RVO:61388963
Klíčová slova: fluorescent nanodiamonds * free radicals * magnetometry * single-particle tracking
Obor OECD: Nano-materials (production and properties)
Impakt faktor: 13.3, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1002/smll.202201395
Diamond magnetometry can provide new insights on the production of free radicals inside live cells due to its high sensitivity and spatial resolution. However, the measurements often lack intracellular context for the recorded signal. In this paper, the possible use of single-particle tracking and trajectory analysis of fluorescent nanodiamonds (FNDs) to bridge that gap is explored. It starts with simulating a set of different possible scenarios of a particle's movement, reflecting different modes of motion, degrees of confinement, as well as shapes and sizes of that confinement. Then, the insights from the analysis of the simulated trajectories are applied to describe the movement of FNDs in glycerol solutions. It is shown that the measurements are in good agreement with the previously reported findings and that trajectory analysis yields meaningful results, when FNDs are tracked in a simple environment. Then the much more complex situation of FNDs moving inside a live cell is focused. The behavior of the particles after different incubation times is analyzed, and the possible intracellular localization of FNDs is deducted from their trajectories. Finally, this approach is combined with long-term magnetometry methods to obtain maps of the spin relaxation dynamics (or T1) in live cells, as FNDs move through the cytosol.
Trvalý link: https://hdl.handle.net/11104/0333631
Počet záznamů: 1