Počet záznamů: 1
Fluorescent Carbon Quantum Dots Functionalized by Poly L-Lysine: Efficient Material for Antibacterial, Bioimaging and Antiangiogenesis Applications
- 1.0560541 - ÚŽFG 2023 RIV US eng J - Článek v odborném periodiku
Vibhute, A. - Nille, O. - Kolekar, G. - Rohiwal, Sonali Suresh - Patil, S. - Lee, S. - Tiwari, A. P.
Fluorescent Carbon Quantum Dots Functionalized by Poly L-Lysine: Efficient Material for Antibacterial, Bioimaging and Antiangiogenesis Applications.
Journal of Fluorescence. Roč. 32, č. 5 (2022), s. 1789-1800. ISSN 1053-0509. E-ISSN 1573-4994
Institucionální podpora: RVO:67985904
Klíčová slova: Carbon quantum dots * Poly 1 lysine * Antibacterial activity * Antiangiogenesis * Bioimaging
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 2.7, rok: 2022
Způsob publikování: Omezený přístup
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=90241735603
This study illustrates the synthesis of functionalized carbon quantum dots (CQDs) by the one-pot pyrolysis method. The functionalization agent used in CQD synthesis was poly 1- lysine (PLL). Various physicochemical techniques were employed to confirm the successful formation of PLLCQD including High resolution transmission electron microscopy (HR-TEM), UV-Vis spectroscopy, fluorescence spectroscopy, Atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy. The size of PLLCQD was confirmed by HRTEM and AFM. The synthesized PLLCQD shows bright blue fluorescence and has a quantum yield of 19.35%. The highest emission band was observed at 471nm when excited to 370nm. The prepared PLLCQD exhibited excellent antibacterial activity against Escherichia coli and Staphylococcus aureus with inhibition zone 7-20 mm. The concentrations of 0.9 to 0.1gmL-1 were studied to determine minimum inhibitory concentration (MIC) by the agar well diffusion assay method. MIC of 0.2gml1 concentration of PLLCQD is achieved. The anti-angiogenic activity of PLLCQD was determined using (Chick Chorioallantoic Membrane) CAM assay. CAM assay is a reliable invivo model to study angiogenesis also, many stimulators and inhibitors have been examined by this method. This study proves higher antibacterial efficiency of PLLCQD over non functionalized CQD. PLLCQD was successfully employed in bio-imaging of the bacterial cell through fluorescence microscopy. Further, PLLCQD displayed cytotoxic effect on endothelial cells and inhibited blood vessel formation in the CAM model.
Trvalý link: https://hdl.handle.net/11104/0333497
Počet záznamů: 1