The Splicing Variant TFIIIA-7ZF of Viroid-Modulated Transcription Factor IIIA Causes Physiological Irregularities in Transgenic Tobacco and Transient Somatic Depression of 'Degradome' Characteristic for Developing Pollen

0559966 - BC 2023 RIV CH eng J - Článek v odborném periodiku
Matoušek, Jaroslav - Steger, G.
The Splicing Variant TFIIIA-7ZF of Viroid-Modulated Transcription Factor IIIA Causes Physiological Irregularities in Transgenic Tobacco and Transient Somatic Depression of 'Degradome' Characteristic for Developing Pollen.
Cells. Roč. 11, č. 5 (2022), č. článku 784. E-ISSN 2073-4409
Grant CEP: GA ČR(CZ) GC18-10515J
Institucionální podpora: RVO:60077344
Klíčová slova: Nicotiana tabacum * viroid * plant transformation * nucleolytic enzymes * plant aging * plant morphology changes * transcription factors
Obor OECD: Genetics and heredity (medical genetics to be 3)
Impakt faktor: 6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2073-4409/11/5/784

Viroids are small, non-coding, pathogenic RNAs with a significant ability of adaptation to several basic cellular processes in plants. TFIIIA-7ZF, a splicing variant of transcription factor IIIA, is involved in replication of nuclear-replicating viroids by DNA-dependent polymerase II. We overexpressed NbTFIIIA-7ZF from Nicotiana benthamiana in tobacco (Nicotiana tabacum) where it caused morphological and physiological deviations like plant stunting, splitting of leaf petioles, pistils or apexes, irregular branching of shoots, formation of double-blade leaves, deformation of main stems, and modification of glandular trichomes. Plant aging and senescence was dramatically delayed in transgenic lines. Factors potentially involved in viroid degradation and elimination in pollen were transiently depressed in transgenic leaves. This depressed degradome in young plants involved NtTudor S-like nuclease, dicers, argonoute 5, and pollen extracellular nuclease I showing expression in tobacco anthers and leaves. Analysis of the degradome in tobacco leaves transformed with either of two hop viroids confirmed modifications of the degradome and TFIIIA expression. Thus, the regulatory network connected to TFIIIA-7ZF could be involved in plant pathogenesis as well as in viroid adaptation to avoid its degradation. These results support the hypothesis on a significant impact of limited TFIIIA-7ZF on viroid elimination in pollen.
Trvalý link: https://hdl.handle.net/11104/0340323