Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?

0559868 - ÚIACH 2023 RIV NL eng J - Článek v odborném periodiku
Dosedělová, Věra - Laštovičková, Markéta - Ayala-Cabrera, J. F. - Dolina, J. - Konečný, S. - Schmitz, O. J. - Kubáň, Petr
Quantification and identification of bile acids in saliva by liquid chromatography-mass spectrometry: Possible non-invasive diagnostics of Barrett's esophagus?
Journal of Chromatography A. Roč. 1676, AUG (2022), č. článku 463287. ISSN 0021-9673. E-ISSN 1873-3778
Institucionální podpora: RVO:68081715
Klíčová slova: IC-MS * saliva analysis * solid-phase extraction
Obor OECD: Analytical chemistry
Impakt faktor: 4.1, rok: 2022
Způsob publikování: Omezený přístup

Bile acids are a group of steroid compounds essential for lipid digestion. However, when bile acids are refluxed into the stomach and the esophagus, during the so called duodenogastroesophageal reflux, they can have a detrimental effect on the esophageal epithelium and cause pathological changes of esophageal tissue, e.g., Barrett's esophagus (BE). The levels of bile acids in saliva could therefore serve as possi-ble biomarkers for the diagnostics of BE. In this work, we focused on optimization of sample collection and preparation by solid-phase extraction and subsequent quantification of 11 bile acids (unconjugated, glycine-conjugated) in saliva from healthy volunteers and BE patients by ultra-high-performance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry. Moreover, high resolution MS (Orbitrap-MS) was utilized for identification of new bile acids in saliva. Methods for saliva collection in-cluding simple spitting and the Salivette (R) saliva collection system were compared, the latter was found to be unsuitable due to excessive retention of bile acids in the cotton swab. Methanol with 0.1% formic acid were selected for protein precipitation and bile acid extraction prior to SPE. Separation was per-formed in gradient elution of methanol and 0.1% formic acid in less than 10 min. Saliva from BE patients contained higher levels of almost all bile acids, and the tested groups could be distinguished by princi-pal component analysis. In untargeted analysis by high resolution MS, taurine-conjugated bile acids and glycine-conjugated dihydroxy-bile acid sulfate were identified in saliva from healthy volunteers. We pro-pose that analysis of salivary bile acids including taurine conjugates could be applicable in diagnostics of BE, following a larger clinical study. (c) 2022 Elsevier B.V. All rights reserved.
Trvalý link: https://hdl.handle.net/11104/0333088