Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach

0559849 - MBÚ 2023 RIV CH eng J - Článek v odborném periodiku
Maťátková, O. - Michailidu, J. - Ježdík, R. - Jarošová Kolouchová, I. - Řezanka, Tomáš - Jirků, V. - Masák, J.
Production and Characterization of Rhamnolipids Produced by Pseudomonas aeruginosa DBM 3774: Response Surface Methodology Approach.
Microorganisms. Roč. 10, č. 7 (2022), č. článku 1272. E-ISSN 2076-2607
Institucionální podpora: RVO:61388971
Klíčová slova: biosurfactants * fractional factorial design * Pseudomonas aeruginosa
Obor OECD: Microbiology
Impakt faktor: 4.5, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/2076-2607/10/7/1272

Rhamnolipids are extensively studied biosurfactants due to their potential in many industrial applications, eco-friendly production and properties. However, their availability for broader application is severely limited by their production costs, therefore the optimization of efficacy of their cultivation gains significance as well as the information regarding the physio-chemical properties of rhamnolipids resulting from various cultivation strategies. In this work, the bioprocess design focused on optimization of the rhamnolipid yield of Pseudomonas aeruginosa DBM 3774 utilizing the response surface methodology (RSM). Six carbon sources were investigated for their effect on the rhamnolipid production. The RSM prediction improved the total rhamnolipid yield from 2.2 to 13.5 g/L and the rhamnolipid productivity from 11.6 to 45.3 mg/L/h. A significant effect of the carbon source type, concentration and the C/N ratio on the composition of the rhamnolipid congeners has been demonstrated for cultivation of P. aeruginosa DBM 3774 in batch cultivation. Especially, changes in presence of saturated fatty acid in the rhamnolipid congeners, ranging from 18.8% of unsaturated fatty acids (carbon source glycerol, 40 g/L) to 0% (sodium citrate 20 g/L) were observed. This demonstrates possibilities of model based systems as basis in cultivation of industrially important compounds like biosurfactants rhamnolipids and the importance of detailed study of interconnection between cultivation conditions and rhamnolipid mixture composition and properties.
Trvalý link: https://hdl.handle.net/11104/0333436