Počet záznamů: 1
Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions
- 1.0559536 - ÚOCHB 2023 RIV DE eng J - Článek v odborném periodiku
Šimonová, Anna - Romanská, Veronika - Benoni, Barbora - Škubník, K. - Šmerdová, L. - Procházková, M. - Spustová, Kristina - Moravčík, Ondřej - Gahurová, Lenka - Pačes, Jan - Plevka, P. - Cahová, Hana
Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions.
Chembiochem. Roč. 23, č. 17 (2022), č. článku e202200281. ISSN 1439-4227. E-ISSN 1439-7633
Grant CEP: GA MŠMT(CZ) LL1603; GA MŠMT(CZ) LM2018131
Institucionální podpora: RVO:61388963 ; RVO:68378050
Klíčová slova: human Rhinovirus type 2 * human Echovirus 18 * LC-MS
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 3.2, rok: 2022
Způsob publikování: Open access
https://doi.org/10.1002/cbic.202200281
The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles, thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC–MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N6-methyladenosine and 2’-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m1A) and 5-methylcytidine (m5C). Mapping of m1A and m5C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3’ and 5’ fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.
Trvalý link: https://hdl.handle.net/11104/0332804
Vědecká data: ENA
Počet záznamů: 1