Versatile compressive microscope for hyperspectral transmission and fluorescence lifetime imaging

0559516 - ÚFP 2023 RIV US eng J - Článek v odborném periodiku
Klein, Lukáš - Kristoffersen, A. S. - Touš, J. - Žídek, Karel
Versatile compressive microscope for hyperspectral transmission and fluorescence lifetime imaging.
Optics Express. Roč. 30, č. 9 (2022), s. 15708-15720. ISSN 1094-4087
Grant CEP: GA MŠMT(CZ) EF16_026/0008390; GA ČR(CZ) GA22-09296S
Institucionální podpora: RVO:61389021
Klíčová slova: microscope * fluorescence * hyperspectral transmissio
Obor OECD: Optics (including laser optics and quantum optics)
Impakt faktor: 3.8, rok: 2022
Způsob publikování: Open access
https://opg.optica.org/oe/fulltext.cfm?uri=oe-30-9-15708&id=471668

Increasing demand for multimodal characterization and imaging of new materials entails the combination of various methods in a single microscopic setup. Hyperspectral imaging of transmission spectra or photoluminescence (PL) decay imaging count among the most used methods. Nevertheless, these methods require very different working conditions and instrumentation. Therefore, combining the methods into a single microscopic system is seldom implemented. Here we demonstrate a novel versatile microscope based on single-pixel imaging, where we use a simple optical configuration to measure the hyperspectral information, as well as fluorescence lifetime imaging (FLIM). The maps are inherently spatially matched and can be taken with spectral resolution limited by the resolution of the used spectrometer (3 nm) or temporal resolution set by PL decay measurement (120 ps). We verify the system’s performance by its comparison to the standard FLIM and non-imaging transmission spectroscopy. Our approach enabled us to switch between a broad field-of-view and micrometer resolution without changing the optical configuration. At the same time, the used design opens the possibility to add a variety of other characterization methods. This article demonstrates a simple, affordable way of complex material studies with huge versatility for the imaging parameters.
Trvalý link: https://hdl.handle.net/11104/0332791