Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation

0559402 - BTÚ 2023 RIV CH eng J - Článek v odborném periodiku
Bosáková, Tereza - Tockstein, A. - Bosáková, Z. - Komrsková, Kateřina
Kinetic Study of 17 alpha-Estradiol Mechanism during Rat Sperm Capacitation.
Molecules. Roč. 27, č. 13 (2022), č. článku 4092. E-ISSN 1420-3049
Grant CEP: GA ČR(CZ) GC20-20217J; GA MŠMT(CZ) ED1.1.00/02.0109
Institucionální podpora: RVO:86652036
Klíčová slova: 17 alpha-estradiol * estrogen receptors * rat * sperm * capacitation * hplc ms/ms * kinetics
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.6, rok: 2022
Způsob publikování: Open access
https://www.mdpi.com/1420-3049/27/13/4092

17 alpha-Estradiol (alpha E2) is a natural diastereoisomer of 17 beta-estradiol (E2). It is well known that alpha E2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of alpha E2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added alpha E2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations B-t were subjected to kinetic analysis. The findings indicated that alpha E2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.
Trvalý link: https://hdl.handle.net/11104/0332928