Selected Triorganotin Compounds in the Absence or Presence of Natural Retinoid Affect Expression of Proteins Associated With Tumour Progression in Human Breast Cancer Mda-mb-231 Cells

0559354 - ÚIACH 2023 SK eng A - Abstrakt
Brtko, J. - Strouhalová, Dana - Toporová, L. - Macejová, D. - Bobálová, Janette
Selected Triorganotin Compounds in the Absence or Presence of Natural Retinoid Affect Expression of Proteins Associated With Tumour Progression in Human Breast Cancer Mda-mb-231 Cells.
European Pharmaceutical Journal. Roč. 69, s1 (2022), s. 34-34. ISSN 1338-6786. E-ISSN 1338-6786.
[Farmakologické dni 2022. 22.06.2022-24.06.2022, Bratislava]
Grant ostatní: AV ČR(CZ) SAV-18-16
Program: Bilaterální spolupráce
Institucionální podpora: RVO:68081715
Klíčová slova: nuclear retinoic acid receptors (RARs) * nuclear retinoid X receptors (RXRs) * MDA-MB-231 cells
Obor OECD: Analytical chemistry

Nuclear retinoic acid receptors (RARs) and nuclear retinoid X receptors (RXRs) are retinoid/rexinoid inducible transcription factors. Trialkyltins and triaryltins, a class of organometallic compounds, function as nuclear RXR agonists due to their capability to bind to the ligand-binding domain of RXR subtypes and function as transcriptional activators. In this study, we present the proteomic data confirming that selected triorganotin compounds affect expression of proteins associated with tumour progression in human breast cancer MDA-MB-231 cells. Proteomic strategies based on a bottom-up method were applied in this study. The total MDA-MB-231 human cell proteins were extracted, separated on 2D SDS-PAGE and their characterization was achieved by MALDI-TOF/TOF MS/MS. Employing PDQuest™ software, we identified more than 30 proteins differently affected by the above compounds. Specific proteins associated either with metabolic pathway (glyceraldehyde-3-phosphate dehydrogenase) or cellular processes such as apoptosis, regulation of gene transcription, or epithelial–mesenchymal transition (annexin 5, nucleoside diphosphate kinase B, and vimentin) have been selected for further analyses. We found that treatment of MDA-MB-231 cells with selected triorganotin compounds reduced the expression of studied proteins. Moreover, the treatment of MDA-MB-231 cells with selected triorganotin compounds together with ATRA resulted in an additional reduction of annexin 5, vimentin, and nucleoside diphosphate kinase B. These results demonstrate that an RXR/RAR heterodimer might act under this experimental design as a permissive heterodimer allowing direct activation of RXRs by triorganotins.
Trvalý link: https://hdl.handle.net/11104/0332694