An Evolutionary and Environmental Perspective of the Interaction of Nanomaterials with the Immune System-The Outcomes of the EU Project PANDORA

0559249 - MBÚ 2023 RIV CH eng M - Část monografie knihy
Navarro Pacheco, Natividad Isabel - Roubalová, Radka - Semerád, Jaroslav - Grasserová, Alena - Benada, Oldřich - Cajthaml, Tomáš - Dvořák, Jiří - Bilej, Martin
In vitro interactions of TiO2 Nanoparticles with earthworm coelomocytes: immunotoxicity assessment. Nanomaterials.
An Evolutionary and Environmental Perspective of the Interaction of Nanomaterials with the Immune System-The Outcomes of the EU Project PANDORA. Basel: MDPI, 2022 - (Boraschi, D.), s. 49-65. ISBN 978-3-0365-3948-5
Institucionální podpora: RVO:61388971
Klíčová slova: earthworm * coelomocyte * TiO2 nanoparticles * reactive oxygen species * innate immunity * lipid peroxidation * alkaline comet assay * phagocytosis * gene expression
Obor OECD: Microbiology
https://www.mdpi.com/books/pdfdownload/book/5502

Titanium dioxide nanoparticles (TiO2 NPs) are manufactured worldwide. Once they arrive in the soil environment, they can endanger living organisms. Hence, monitoring and assessing the effects of these nanoparticles is required. We focus on the Eisenia andrei earthworm immune cells exposed to sublethal concentrations of TiO2 NPs (1, 10, and 100 μg/mL) for 2, 6, and 24 h. TiO2 NPs at all concentrations did not affect cell viability. Further, TiO2 NPs did not cause changes in reactive
oxygen species (ROS) production, malondialdehyde (MDA) production, and phagocytic activity. Similarly, they did not elicit DNA damage. Overall, we did not detect any toxic effects of TiO2 NPs at the cellular level. At the gene expression level, slight changes were detected. Metallothionein, fetidin/lysenin, lumbricin and MEK kinase I were upregulated in coelomocytes after exposure to 10 μg/mL TiO2 NPs for 6 h. Antioxidant enzyme expression was similar in exposed and control cells. TiO2 NPs were detected on coelomocyte membranes. However, our results do not show any strong effects of these nanoparticles on coelomocytes at both the cellular and molecular levels.
Trvalý link: https://hdl.handle.net/11104/0332587