Počet záznamů: 1  

Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus)

  1. 1.
    0558972 - BTÚ 2023 RIV CH eng J - Článek v odborném periodiku
    Gazo, I. - Naraine, Ravindra - Lebeda, I. - Tomcala, Al. - Dietrich, M. - Franěk, R. - Pšenička, M. - Šindelka, Radek
    Transcriptome and Proteome Analyses Reveal Stage-Specific DNA Damage Response in Embryos of Sturgeon (Acipenser ruthenus).
    International Journal of Molecular Sciences. Roč. 23, č. 12 (2022), č. článku 6392. E-ISSN 1422-0067
    Grant CEP: GA ČR(CZ) GA19-11313S
    Institucionální podpora: RVO:86652036
    Klíčová slova: fish * sturgeon * embryo * DNA damage response * omics
    Obor OECD: Biochemistry and molecular biology
    Impakt faktor: 5.6, rok: 2022
    Způsob publikování: Open access
    https://www.mdpi.com/1422-0067/23/12/6392

    DNA damage during early life stages may have a negative effect on embryo development, inducing mortality and malformations that have long-lasting effects during adult life. Therefore, in the current study, we analyzed the effect of DNA damage induced by genotoxicants (camptothecin (CPT) and olaparib) at different stages of embryo development. The survival, DNA fragmentation, transcriptome, and proteome of the endangered sturgeon Acipenser ruthenus were analyzed. Sturgeons are non-model fish species that can provide new insights into the DNA damage response and embryo development. The transcriptomic and proteomic patterns changed significantly after exposure to genotoxicants in a stage-dependent manner. The results of this study indicate a correlation between phenotype formation and changes in transcriptomic and proteomic profiles. CPT and olaparib downregulated oxidative phosphorylation and metabolic pathways, and upregulated pathways involved in nucleotide excision repair, base excision repair, and homologous recombination. We observed the upregulated expression of zona pellucida sperm-binding proteins in all treatment groups, as well as the upregulation of several glycolytic enzymes. The analysis of gene expression revealed several markers of DNA damage response and adaptive stress response, which could be applied in toxicological studies on fish embryos. This study is the first complex analysis of the DNA damage response in endangered sturgeons.
    Trvalý link: https://hdl.handle.net/11104/0332508

     
     
Počet záznamů: 1  

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