Expression of genes encoding carbohydrate-metabolic enzymes during taproot development in sugar beet (Beta vulgaris subsp. vulgaris)

0558511 - ÚVGZ 2023 RIV AT eng J - Článek v odborném periodiku
Jammer, A. - Albacete, A. - Schulz, B. - Koch, W. - Weltmeier, F. - van der Graaff, E. - Roitsch, Thomas
Expression of genes encoding carbohydrate-metabolic enzymes during taproot development in sugar beet (Beta vulgaris subsp. vulgaris).
Phyton. Annales Rei Botanicae. Roč. 61, DEC (2021), s. 31-50. ISSN 0079-2047
Grant CEP: GA MŠMT(CZ) LO1415
Institucionální podpora: RVO:86652079
Klíčová slova: organ-specific expression * sucrose-cleaving enzymes * invertase activity * translocation * anatomy * defense * storage * yield * Beta vulgaris subsp. vulgaris * assimilate partitioning * carbohydrate metabolism * developmental regulation * taproot development * sucrose accumulation
Obor OECD: Plant sciences, botany
Impakt faktor: 0.360, rok: 2021
Způsob publikování: Open access
https://www.verlag-berger.at/detailview?no=2974

In sugar beet (Beta vulgaris subsp. vulgaris, Chenopodiaceae), sucrose is stored in a taproot developed by a genus-specific type of anomalous secondary thickening from supernumerary cambial rings. In a previous study (Jammer & al. 2020), we showed that the transition from primary root development to the formation of the sucrose-storing taproot is accompanied by a clear shift in physiological signatures: characteristic changes in the activities of several key enzymes of carbohydrate metabolism were associated with the onset of taproot development and sucrose storage, resulting in three distinct physiological stages (prestorage, transition, secondary growth and sucrose storage). In order to investigate the regulatory mechanisms behind these changes in enzyme activities, we performed a microarray expression analysis for the genes encoding the respective carbohydrate-metabolic enzymes. The data obtained from the microarray were validated for selected transcripts by semi-quantitative reverse transcription PCR. A high level of similarity between the developmental changes in transcript levels and enzyme activities at the transition stage suggests that the majority of the respective enzymes are predominantly regulated on the transcriptional level during the early stages of taproot development.
Trvalý link: http://hdl.handle.net/11104/0332149